Surdin-Kerjan Y, Cherest H, Robichon-Szulmajster H
J Bacteriol. 1973 Mar;113(3):1156-60. doi: 10.1128/jb.113.3.1156-1160.1973.
Derepression of some methionine biosynthetic enzymes (methionine group I enzymes) obtained in methionine limitation has been found to be accompanied by a significant lack of in vivo charging of bulk methionine transfer ribonucleic acid (tRNA(Met)) and in addition by a decreased rate of synthesis of all tRNAs. Under the same conditions, methionyl-tRNA synthetase (MTS) was derepressed rather than repressed. These results are in agreement with those previously published based on studies of a mutant with an impaired MTS (5) and reinforce the idea that the rate of synthesis of methionine group I enzymes can be related to the total content of methionyl (Met)-tRNA (Met) per cell. They also render unlikely that MTS could be a constituent of the regulatory signal.
在蛋氨酸限制条件下获得的一些蛋氨酸生物合成酶（第一组蛋氨酸酶）的去阻遏，已被发现伴随着大量蛋氨酸转运核糖核酸（tRNA(Met)）在体内显著缺乏充电，此外还伴随着所有tRNA合成速率的降低。在相同条件下，甲硫氨酰-tRNA合成酶（MTS）被去阻遏而非被抑制。这些结果与先前基于对MTS受损的突变体的研究发表的结果一致（5），并强化了这样一种观点，即第一组蛋氨酸酶的合成速率可能与每个细胞中甲硫氨酰（Met）-tRNA（Met）的总含量有关。它们还使得MTS不太可能成为调节信号的组成部分。
