McGinnis E, Williams L S
J Bacteriol. 1972 Sep;111(3):739-44. doi: 10.1128/jb.111.3.739-744.1972.
Control of formation of the histidyl-transfer ribonucleic acid (tRNA) synthetase with an increased K(m) for histidine was studied in a hisS mutant of Salmonella typhimurium. Histidine restriction of both the hisS and hisS(+) strains resulted in a derepression of synthesis of histidyl-tRNA synthetase. When grown in a concentration less than the K(m) (100 mug/ml) of l-histidine, the hisS mutant maintained a higher level of histidyl-tRNA synthetase than the hisS(+) strain. Addition of excess amounts of l-histidine to the growth medium of the hisS mutant culture grown with 100 mug of l-histidine per ml resulted in a repression of histidyl-tRNA synthetase formation to equal that of the hisS(+) strain grown in 100 mug of l-histidine per ml. These data confirm previous findings that histidine tRNA is involved in the repression of synthesis of histidyl-tRNA synthetase.
在鼠伤寒沙门氏菌的hisS突变体中，对组氨酸的米氏常数（Km）增加的组氨酰 - 转移核糖核酸（tRNA）合成酶的形成控制进行了研究。对hisS和hisS(+)菌株进行组氨酸限制导致组氨酰 - tRNA合成酶合成的去阻遏。当在浓度低于L - 组氨酸的Km（100μg/ml）下生长时，hisS突变体维持的组氨酰 - tRNA合成酶水平高于hisS(+)菌株。向每毫升含有100μg L - 组氨酸生长的hisS突变体培养物的生长培养基中添加过量的L - 组氨酸，导致组氨酰 - tRNA合成酶形成受到抑制，与每毫升含有100μg L - 组氨酸生长的hisS(+)菌株的抑制程度相同。这些数据证实了先前的发现，即组氨酸tRNA参与组氨酰 - tRNA合成酶合成的阻遏。
