Brittinger G, Augener W, Bremer K, Cohnen G, Dabag S, Fischer K, König E, Meusers P
Blut. 1975 May;30(5):293-308. doi: 10.1007/BF01633649.
In patients with chronic lymphatic leukemia (CLL) the majority of peripheral blood lymphocytes was characterized as B cells by surface membrane markers. Ultrastructural studies revealed a reduction of the cytoplasmic area. Furthermore, the number of lysosomes was diminished corresponding to a decreased activity of lysosomal hydrolases. Increasing blood lymphocytosis was paralleled by an increase of the percentage of lysosome-poor lymphocytes. Response of CLL lymphocytes to in vitro stimulation with PHA and PWM was either lacking or diminished and/or delayed, and the number of transformed cells was reduced. Thus, the majority of CLL lymphocytes appears to represent both morphologically and functionally abnormal neoplastic b cells. During the early and later phase of stimulation the mitogen-reactive CLL lymphocytes exhibited alterations of the lysosomal apparatus similar to those observed in normal cells. The reactive lymphocytes may be derived from residual populations of normally functioning T and/or B cells. However, the neoplastic cells may also be able to respond to the mitogens. In vivo studies showed impaired kinetics of circulation and recirculation of CLL B lymphocytes, whereas the T cells were normal in this respect.
在慢性淋巴细胞白血病（CLL）患者中，大多数外周血淋巴细胞通过表面膜标志物被鉴定为B细胞。超微结构研究显示细胞质面积减少。此外，溶酶体数量减少，这与溶酶体水解酶活性降低相对应。血液淋巴细胞增多的同时，缺乏溶酶体的淋巴细胞百分比增加。CLL淋巴细胞对PHA和PWM体外刺激的反应要么缺乏、减弱和/或延迟，并且转化细胞数量减少。因此，大多数CLL淋巴细胞在形态和功能上似乎都代表异常的肿瘤性B细胞。在刺激的早期和后期，有丝分裂原反应性CLL淋巴细胞表现出与正常细胞中观察到的类似的溶酶体装置改变。反应性淋巴细胞可能来源于正常功能的T和/或B细胞的残余群体。然而，肿瘤细胞也可能能够对有丝分裂原产生反应。体内研究表明CLL B淋巴细胞的循环和再循环动力学受损，而T细胞在这方面是正常的。
