Van Den Hondel C A, Schoenmakers J G
Eur J Biochem. 1975 May 6;53(2):547-58. doi: 10.1111/j.1432-1033.1975.tb04098.x.
A physical map of the bacteriophage M13 genome has been constructed on the basis of specific cleavage of M13 replicative form DNA by bacterial restriction endonucleases. The 13 fragments produced by the enzyme from Haemophilus aphirophilus (endonuclease R.Hap II) as well as the 10 fragments produced by the enzyme from Haemophilus aegyptius (endonuclease R.Hae III) have been ordered by analysis of partial digest products and by analysis of overlapping sets of fragments. In addition, the single site in M13 replicative form DNA cleaved by the restriction enzyme from Haemophilus influenzae Rd (endonuclease R.Hin dII) has been located more precisely. With this unique site as a reference point, the H. aphirophilus cleavage sites and the H. aegyptius cleavage sites have been localized on the map.
基于细菌限制性内切核酸酶对M13复制型DNA的特异性切割，构建了噬菌体M13基因组的物理图谱。通过对部分消化产物的分析以及对重叠片段组的分析，已将嗜沫嗜血杆菌（内切核酸酶R.Hap II）产生的13个片段以及埃及嗜血杆菌（内切核酸酶R.Hae III）产生的10个片段进行了排序。此外，已更精确地定位了流感嗜血杆菌Rd（内切核酸酶R.Hin dII）切割的M13复制型DNA中的单个位点。以这个独特的位点作为参考点，嗜沫嗜血杆菌的切割位点和埃及嗜血杆菌的切割位点已在图谱上定位。
