Engelberg-Kulka H, Dekel L, Israeli-Reches M, Belfort M
Mol Gen Genet. 1979 Feb 26;170(2):155-9. doi: 10.1007/BF00337791.
A spontaneous streptomycin-resistant Escherichia coli mutant which is temperature-sensitive for suppression of a nonsense codon was studied for its ability to propagate phages T2, T4D, T5, phi K, f2, MS2, R17, Q beta, lambda as well as filamentous phages fl, fd and M13. Of all phages tested, only the growth of Q beta, lambda, and filamentous phages is inhibited in the mutant at 42 degree C. This selective inhibition suggests that, like Q beta, lambda and filamentous phages also require a read-through proten(s) which results from suppression of a termination codon.
研究了一株对无义密码子抑制呈温度敏感的自发链霉素抗性大肠杆菌突变体，以考察其对噬菌体T2、T4D、T5、φK、f2、MS2、R17、Qβ、λ以及丝状噬菌体fl、fd和M13的增殖能力。在所有测试的噬菌体中，只有Qβ、λ和丝状噬菌体在该突变体中于42℃时生长受到抑制。这种选择性抑制表明，与Qβ一样，λ和丝状噬菌体也需要一种由终止密码子抑制产生的通读蛋白。
