Purdy D, Buswell C M, Hodgson A E, McALPINE K, Henderson I, Leach S A
Centre for Applied Microbiology and Research, Salisbury, Wiltshire, SP4 0JG.
J Med Microbiol. 2000 May;49(5):473-479. doi: 10.1099/0022-1317-49-5-473.
In order to assess the contribution of cytolethal distending toxin (CDT) to the toxigenicity and pathogenicity of Campylobacter jejuni, the C. jejuni 81-176 and C. jejuni NCTC 11168 CDTs were inactivated by insertional mutation of the cdtB toxin subunit. Cell-free sonicates from isogenic C. jejuni 81-176 cdtB- strains were found to be greatly attenuated in HeLa cytotoxicity assays, whilst still retaining some toxigenicity. Sonicates from a C. jejuni NCTC 11168 cdtB- strain produced no detectable cytotoxicity. When orally administered to adult severe combined immunodeficient (SCID) mice, C. jejuni cdtB mutant strains were unaffected in enteric colonisation abilities but demonstrated impaired invasiveness into blood, spleen and liver tissues. These data suggest that CDT may be the principal toxin produced by this species and that some C. jejuni strains may generate additional toxigenic factor(s) distinct from CDT.
为了评估细胞致死性膨胀毒素（CDT）对空肠弯曲菌产毒性和致病性的贡献，通过对cdtB毒素亚基进行插入突变，使空肠弯曲菌81 - 176株和空肠弯曲菌NCTC 11168株的CDT失活。在HeLa细胞毒性试验中发现，来自同基因空肠弯曲菌81 - 176 cdtB - 菌株的无细胞超声裂解物的细胞毒性大大减弱，同时仍保留一些产毒性。来自空肠弯曲菌NCTC 11168 cdtB - 菌株的超声裂解物未产生可检测到的细胞毒性。当给成年重度联合免疫缺陷（SCID）小鼠口服时，空肠弯曲菌cdtB突变株的肠道定植能力未受影响，但对血液、脾脏和肝脏组织的侵袭能力受损。这些数据表明，CDT可能是该菌种产生的主要毒素，并且一些空肠弯曲菌菌株可能产生不同于CDT的其他产毒因子。
