Ryan K J, Wente S R
Department of Cell Biology and Physiology, Washington University School of Medicine, Box 8228, St Louis, MO 63110, USA. kryan@cellbio. wustl.edu
Curr Opin Cell Biol. 2000 Jun;12(3):361-71. doi: 10.1016/s0955-0674(00)00101-0.
Compositional analysis of nuclear pore complexes (NPCs) is nearing completion, and efforts are now focused on understanding how these protein machines work. Recent analysis of soluble transport factor interactions with NPC proteins reveals distinct and overlapping pathways for movement between the nucleus and cytoplasm. New fluorescence- and microscopy-based strategies have been used to monitor the pathway of NPC assembly and to reveal the dynamics of the NPC during transport.
核孔复合体（NPCs）的成分分析已接近完成，目前的工作重点是了解这些蛋白质机器是如何运作的。最近对可溶性转运因子与NPC蛋白相互作用的分析揭示了细胞核与细胞质之间不同且重叠的转运途径。基于荧光和显微镜的新策略已被用于监测NPC组装途径并揭示其在转运过程中的动态变化。
