X射线使转化脱氧核糖核酸失活的机制。
Mechanism of inactivation of transforming deoxyribonucleic acid by X rays.
作者信息
Randolph M L, Setlow J K
出版信息
J Bacteriol. 1971 Apr;106(1):221-6. doi: 10.1128/jb.106.1.221-226.1971.
Abstract
Transforming deoxyribonucleic acid (DNA) from Haemophilus influenzae was exposed to X rays either in phosphate buffer or in 10% yeast extract. Relations between determinations of biological inactivation, DNA uptake by competent H. influenzae, integration of DNA into the competent cell genome, and induced single-and double-strand breaks indicate that transforming DNA is inactivated by the direct and the indirect effect of X radiation primarily because integration of DNA is prevented as a result of the production of double-strand breaks.
摘要
来自流感嗜血杆菌的转化脱氧核糖核酸（DNA）在磷酸盐缓冲液或10%酵母提取物中接受X射线照射。生物失活测定、感受态流感嗜血杆菌对DNA的摄取、DNA整合到感受态细胞基因组以及诱导的单链和双链断裂之间的关系表明，转化DNA因X射线的直接和间接作用而失活，主要是因为双链断裂的产生阻止了DNA的整合。
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