Kantor G J, Barnhart B J
J Bacteriol. 1973 Mar;113(3):1228-34. doi: 10.1128/jb.113.3.1228-1234.1973.
The wild-type strain and mutants of Haemophilus influenzae, sensitive or resistant to ultraviolet light (UV) as defined by colony-forming ability, were examined for their ability to perform the incision and rejoining steps of the deoxyribonucleic acid (DNA) dark repair process. Although UV-induced pyrimidine dimers are excised by the wild-type Rd and a resistant mutant BC200, the expected single-strand DNA breaks could not be detected on alkaline sucrose gradients. Repair of the gap resulting from excision must be rapid when experimental conditions described by us are employed. Single-strand DNA breaks were not detected in a UV-irradiated sensitive mutant (BC100) incapable of excising pyrimidine dimers, indicating that this mutant may be defective in a dimer-recognizing endonuclease. No single-strand DNA breaks were detected in a lysogen BC100(HP1c1) irradiated with a UV dose large enough to induce phage development in 80% of the cells.
对流感嗜血杆菌的野生型菌株及其对紫外线（UV）敏感或耐药的突变体（根据菌落形成能力定义）进行了检测，以评估它们执行脱氧核糖核酸（DNA）暗修复过程中切割和重新连接步骤的能力。尽管野生型Rd和耐药突变体BC200可切除紫外线诱导的嘧啶二聚体，但在碱性蔗糖梯度上未检测到预期的单链DNA断裂。当采用我们描述的实验条件时，切除产生的缺口修复必定很快。在不能切除嘧啶二聚体的紫外线照射敏感突变体（BC100）中未检测到单链DNA断裂，这表明该突变体可能在二聚体识别内切核酸酶方面存在缺陷。在用足以使80%的细胞诱导噬菌体发育的紫外线剂量照射的溶原菌BC100（HP1c1）中未检测到单链DNA断裂。
