Sandberg A L, Wahl S M, Mergenhagen S E
J Immunol. 1975 Jul;115(1):139-44.
Trypsin cleavage of guinea pig C3 (60 sec, 20 degrees C) yields a fragment which stimulates guinea pig lymphocytes to produce a chemotactic lymphokine. These digested C3 preparations possess only minimal inherent chemotactic properties. The response is completely inhibited by prior adsorption of the trypsin-derived C3 fragments with insolubilized anti-C3. The active C3 fragment is C3b as demonstrated by the elution of activity in the exclusion volume of Sephadex G-75 (m.w. greater than 50,000 dalton). The C3b-dependent cellular-derived chemotactic factor is present in the supernatants of B cell cultures and totally absent in those of T cell cultures. Generation of this lymphokine occurs in the absence of cellular proliferation.
用胰蛋白酶裂解豚鼠补体C3（20℃，60秒）可产生一个片段，该片段能刺激豚鼠淋巴细胞产生趋化性淋巴因子。这些经消化的C3制剂仅具有极小的固有趋化特性。用不溶性抗C3预先吸附胰蛋白酶衍生的C3片段可完全抑制该反应。如在Sephadex G - 75排阻体积中洗脱活性所证明的，活性C3片段为C3b（分子量大于50,000道尔顿）。依赖C3b的细胞源性趋化因子存在于B细胞培养上清液中，而在T细胞培养上清液中则完全不存在。这种淋巴因子的产生在无细胞增殖的情况下发生。
