Postlethwaite A E, Kang A H
J Immunol. 1980 Mar;124(3):1462-6.
An assay that measures guinea pig fibroblast chemotaxis in vitro is described. Application of this assay has revealed that stimulation of guinea pig blood or lymph node lymphocytes in vitro by T cell antigen (horseradish peroxidase) or mitogen (phytohemagglutinin) is associated with production of a chemoattractant for guinea pig fibroblasts. This lymphocyte-derived chemotactic factor for fibroblasts (LDCF-F) has a m.w. or 80,000 and is distinct from guinea pig lymphocyte-derived chemotactic factor for monocytes (LDCF-M). LDCF-F is heat labile and trypsin sensitive. The production of LDCF-F in vitro correlates with lymphocyte transformation to antigen or mitogen and with the presence of in vivo-delayed hypersensitivity to antigen. LDCF-F might function in vivo to attract connective tissue fibroblasts to sites of cell-mediated immune reactions.
本文描述了一种体外检测豚鼠成纤维细胞趋化性的试验方法。应用该试验方法发现，用T细胞抗原（辣根过氧化物酶）或丝裂原（植物血凝素）在体外刺激豚鼠血液或淋巴结淋巴细胞，会产生一种对豚鼠成纤维细胞具有趋化作用的物质。这种来源于淋巴细胞的成纤维细胞趋化因子（LDCF-F）分子量为80,000，与豚鼠淋巴细胞来源的单核细胞趋化因子（LDCF-M）不同。LDCF-F对热不稳定，对胰蛋白酶敏感。体外LDCF-F的产生与淋巴细胞对抗原或丝裂原的转化以及体内对抗原的迟发型超敏反应的存在相关。LDCF-F在体内可能发挥作用，将结缔组织成纤维细胞吸引到细胞介导的免疫反应部位。
