Lasserre J, Maury J, Lefevre G, Max-Audit I, Bimet C, Maier-Redelsperger M, Etienne J, Rain J D, Adam E, Benbunan M, Girot R
ETS de l'Assistance publique-Hôpitaux de Paris, France.
Transfus Clin Biol. 2000 Apr;7(2):119-28. doi: 10.1016/s1246-7820(00)88942-9.
In order to evaluate the feasibility of the autologous transfusion in an alloimmunized sickle cell patient, changes in the hematologic and biochemical characteristics of erythrocytes stored for 42 days from two patients with sickle cell SC anemia were compared with control subjects' (Hb A) red blood cells. Erythrocytes were stored in Saline Adenosine Dextrose Mannitol at +4 degrees C. The cryopreservation storage was made and 51Cr red cell survival was measured in one patient. No significant difference in the hematologic and biochemical parameters of the SC red blood cells and the control subjects was observed during the storage at +4 degrees C. Red cell survivals determined in fresh cells, cells stored for 42 days at +4 degrees C and thawed cells from one patient demonstrate much shorter half-life values than those of normal red blood cells. Before application, our results need to be confirmed by the same protocol with another patient with sickle cell SC.
为了评估自体输血在同种免疫的镰状细胞病患者中的可行性，将两名镰状细胞SC贫血患者储存42天的红细胞的血液学和生化特性变化与对照受试者（Hb A）的红细胞进行了比较。红细胞保存在4℃的生理盐水-腺苷-葡萄糖-甘露醇中。对一名患者进行了冷冻保存，并测定了51Cr红细胞存活率。在4℃储存期间，未观察到SC红细胞与对照受试者的血液学和生化参数有显著差异。在一名患者中，新鲜细胞、在4℃储存42天的细胞和解冻细胞的红细胞存活率显示出比正常红细胞短得多的半衰期值。在应用之前，我们的结果需要通过相同方案在另一名镰状细胞SC患者中得到证实。
