Kanayama K, Osada H
Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
J Int Med Res. 2000;28(1):20-3. doi: 10.1177/147323000002800103.
Follicular growth and ovulation were induced in mice by administration of pregnant mare serum gonadotrophin (PMSG) followed 2 days later by human chorionic gonadotrophin; the day of PMSG injection was designated as day 0. The volume of the ampulla was measured and the location of the ovulated eggs determined at 06:00, 10:00, 14:00, 18:00 and 22:00 on day 3, and at 02:00 and 06:00 on day 4. The volume of the ampulla and hence oviductal fluid, peaked at 14:00 on day 3 and then declined. In all oviduct samples taken up to 14:00 on day 3, eggs were found exclusively in the ampulla. Thereafter, an increasing number of eggs were observed in the isthmus. Thus, the migration of eggs from the ampulla to the isthmus was concurrent with the decrease in oviductal fluid volume. The peak in the volume of oviductal fluid seen at day 3 is likely to coincide with the opening of the ampullary-isthmic junction of the oviduct.
通过给小鼠注射孕马血清促性腺激素（PMSG）诱导卵泡生长和排卵，2天后再注射人绒毛膜促性腺激素；PMSG注射日被指定为第0天。在第3天的06:00、10:00、14:00、18:00和22:00以及第4天的02:00和06:00测量壶腹部的体积，并确定排卵卵子的位置。壶腹部的体积以及由此推断的输卵管液体积在第3天的14:00达到峰值，然后下降。在第3天14:00之前采集的所有输卵管样本中，卵子仅在壶腹部被发现。此后，在峡部观察到的卵子数量越来越多。因此，卵子从壶腹部向峡部的迁移与输卵管液体积的减少同时发生。第3天观察到的输卵管液体积峰值可能与输卵管壶腹 - 峡部连接处的开放时间一致。
