Charon N W, Johnson R C, Muschel L H
Infect Immun. 1975 Dec;12(6):1386-91. doi: 10.1128/iai.12.6.1386-1391.1975.
Normal serum from the painted turtle (Chrysemys picta), the snapping turtle (Chelydra serpentina), and the frog (Rana pipiens) were found to possess bactericidal activity towards Leptospira. Leptospires from both the parasitic and biflexa complexes were killed by these sera at high dilutions. This pattern differs from that of mammalian serum, as generally only the biflexa complex leptospires are killed by normal mammalian serum. The activity in C. picta serum was characterized as being complement dependent and not mediated by basic proteins. Because comple-inactivated C. picta serum regained leptospiricidal activity after the addition of fresh rabbit serum, antibody is also likely to participate in the killing activity. Further support that C. picta serum contained leptospiral antibodies was found by the detection of serotype-specific agglutinins.
人们发现，锦龟（彩龟）、鳄龟和青蛙的正常血清对钩端螺旋体具有杀菌活性。来自寄生复合体和双曲钩端螺旋体复合体的钩端螺旋体在这些血清的高稀释度下会被杀死。这种模式与哺乳动物血清不同，因为通常只有双曲钩端螺旋体复合体的钩端螺旋体被正常哺乳动物血清杀死。锦龟血清中的活性被表征为补体依赖性，而非由碱性蛋白介导。由于补体失活的锦龟血清在添加新鲜兔血清后恢复了杀钩端螺旋体活性，抗体也可能参与了杀伤活性。通过检测血清型特异性凝集素，进一步证实锦龟血清中含有钩端螺旋体抗体。
