Belokrylov G A
Zh Mikrobiol Epidemiol Immunobiol. 1976 Apr(3):23-8.
The cytotoxic test was used to assess the specificity of the action of antisera obtained by immunization of rabbits with the tissue of mouse brain, mouse intact lymphoid cells of various origin, and cells preliminarily filtered through a column of cotton. Anticerebral sera proved to react strictly specifically with the thymus-dependent population of lymphoid cells; antithymus sera behaved in analogous way both against the intact and against the filtered thymocytes. The action spectrum of the sera against the cells of the lymph nodes and the spleen depended on the methof obtained them. The sera against the intact cells of the lymph nodes reacted chiefly with the T-cells, killing 20% of the cells of bone marrow origin. The sera against the lymph node cells enriched by filtration were strictly specific; in comparison with the sera against crude cells, the sera against filtered splenic cells contained much more antibodies reacting with T-cells; admixture of antibodies against B cells was the same in the sera against crude and against filtered cells. Immunization of rabbits with mouse bone marrow cells permitted to obtain antiserum which gave a strictly specific reaction with B-cells after a corresponding absorption with thymocytes.
细胞毒性试验用于评估通过用小鼠脑组织、各种来源的完整小鼠淋巴细胞以及预先通过棉柱过滤的细胞免疫兔子所获得的抗血清作用的特异性。抗脑血清被证明与胸腺依赖性淋巴细胞群体发生严格特异性反应；抗胸腺血清对完整的和过滤后的胸腺细胞表现出类似的反应。血清对淋巴结和脾脏细胞的作用谱取决于获得它们的方法。针对淋巴结完整细胞的血清主要与T细胞反应，杀死20%的骨髓来源细胞。针对通过过滤富集的淋巴结细胞的血清具有严格的特异性；与针对粗细胞的血清相比，针对过滤后的脾细胞的血清含有更多与T细胞反应的抗体；针对粗细胞和过滤后细胞的血清中针对B细胞的抗体混合物相同。用小鼠骨髓细胞免疫兔子可获得抗血清，该抗血清在与胸腺细胞进行相应吸收后与B细胞发生严格特异性反应。
