V型ATP酶质子泵的机械化学
The mechanochemistry of V-ATPase proton pumps.
作者信息
Grabe M, Wang H, Oster G
机构信息
Departments of Physics, University of California, Berkeley 94720-3112, USA.
出版信息
Biophys J. 2000 Jun;78(6):2798-813. doi: 10.1016/S0006-3495(00)76823-8.
Abstract
The vacuolar H(+)-ATPases (V-ATPases) are a universal class of proton pumps that are structurally similar to the F-ATPases. Both protein families are characterized by a membrane-bound segment (V(o), F(o)) responsible for the translocation of protons, and a soluble portion, (V(1), F(1)), which supplies the energy for translocation by hydrolyzing ATP. Here we present a mechanochemical model for the functioning of the V(o) ion pump that is consistent with the known structural features and biochemistry. The model reproduces a variety of experimental measurements of performance and provides a unified view of the many mechanisms of intracellular pH regulation.
摘要
液泡H(+) -ATP酶（V -ATP酶）是一类普遍存在的质子泵，在结构上与F -ATP酶相似。这两个蛋白质家族的特点是都有一个负责质子转运的膜结合部分（V(o)，F(o)），以及一个通过水解ATP为转运提供能量的可溶性部分（V(1)，F(1)）。在此，我们提出了一个与已知结构特征和生物化学一致的V(o)离子泵功能的机械化学模型。该模型再现了各种性能的实验测量结果，并为细胞内pH调节的多种机制提供了统一的观点。
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