Nadjar D, Rouveau M, Verdet C, Donay L, Herrmann J, Lagrange P H, Philippon A, Arlet G
Service de Bactériologie, Hôpital Tenon, Paris, France.
FEMS Microbiol Lett. 2000 Jun 1;187(1):35-40. doi: 10.1111/j.1574-6968.2000.tb09133.x.
Fifty-two strains of Klebsiella pneumoniae producing an AmpC-type plasmid-mediated beta-lactamase were isolated from 13 patients in the same intensive care unit between March 1998 and February 1999. These strains were resistant to ceftazidime, cefotaxime and ceftriaxone, but susceptible to cefoxitin, cefepime and aztreonam. Plasmid content and genomic DNA restriction pattern analysis suggested dissemination of a single clone. Two beta-lactamases were identified, TEM-1 and ACC-1. We used internal bla(ACC-1) primers, to sequence PCR products obtained from two unrelated strains of Hafnia alvei. Our results show that the ACC-1 beta-lactamase was derived from the chromosome-encoded AmpC-type enzyme of H. alvei.
1998年3月至1999年2月期间，从同一重症监护病房的13名患者中分离出52株产AmpC型质粒介导β-内酰胺酶的肺炎克雷伯菌。这些菌株对头孢他啶、头孢噻肟和头孢曲松耐药，但对头孢西丁、头孢吡肟和氨曲南敏感。质粒含量和基因组DNA限制性图谱分析提示单一克隆的传播。鉴定出两种β-内酰胺酶，即TEM-1和ACC-1。我们使用内部bla(ACC-1)引物对从两株无关的蜂房哈夫尼亚菌中获得的PCR产物进行测序。我们的结果表明，ACC-1β-内酰胺酶源自蜂房哈夫尼亚菌染色体编码的AmpC型酶。
