Shu Q, Lin H, Rutherfurd K J, Fenwick S G, Prasad J, Gopal P K, Gill H S
Milk and Health Research Centre, Massey University, Palmerston North, New Zealand.
Microbiol Immunol. 2000;44(4):213-22. doi: 10.1111/j.1348-0421.2000.tb02486.x.
The ability of a newly identified probiotic lactic acid bacterial strain, Bifidobacterium lactis (HN019), to confer protection against Salmonella typhimurium was investigated in BALB/c mice. Feeding mice with B. lactis conferred a significant degree of protection against single or multiple oral challenge with virulent S. typhimurium, in comparison to control mice that did not receive B. lactis. Protection included a ten-fold increase in survival rate, significantly higher post-challenge food intake and weight gain, and reduced pathogen translocation to visceral tissues (spleen and liver). Furthermore, the degree of pathogen translocation showed a significant inverse correlation with splenic lymphocyte proliferative responses to mitogens, blood and peritoneal cell phagocytic activity and intestinal mucosal anti-S. typhimurium antibody titers in infected mice; all of these immune parameters were enhanced in mice fed B. lactis. Together, these results suggest that dietary B. lactis can provide a significant degree of protection against Salmonella infection by enhancing various parameters of immune function that are relevant to the immunological control of salmonellosis. Thus dietary supplementation with B. lactis provides a unique opportunity for developing immune-enhancing probiotic dairy food products with proven health benefits.
在BALB/c小鼠中研究了新鉴定的益生菌乳酸菌菌株——乳酸双歧杆菌（HN019）对鼠伤寒沙门氏菌的保护能力。与未接受乳酸双歧杆菌的对照小鼠相比，用乳酸双歧杆菌喂养小鼠可对强毒力鼠伤寒沙门氏菌的单次或多次口服攻击提供显著程度的保护。保护作用包括存活率提高十倍、攻毒后食物摄入量和体重增加显著更高，以及病原体向内脏组织（脾脏和肝脏）的易位减少。此外，病原体易位程度与感染小鼠脾脏淋巴细胞对丝裂原的增殖反应、血液和腹腔细胞吞噬活性以及肠道黏膜抗鼠伤寒沙门氏菌抗体滴度呈显著负相关；在喂食乳酸双歧杆菌的小鼠中，所有这些免疫参数均得到增强。总之，这些结果表明，膳食中的乳酸双歧杆菌可通过增强与沙门氏菌病免疫控制相关的各种免疫功能参数，对沙门氏菌感染提供显著程度的保护。因此，膳食补充乳酸双歧杆菌为开发具有已证实健康益处的免疫增强型益生菌乳制品提供了独特机会。
