Chiang B L, Sheih Y H, Wang L H, Liao C K, Gill H S
College of Medicine, National Taiwan University, Taipei, Taiwan.
Eur J Clin Nutr. 2000 Nov;54(11):849-55. doi: 10.1038/sj.ejcn.1601093.
To define the cellular basis for immune enhancement by a probiotic lactic acid bacteria strain (Bifidobacterium lactis HN019); and to determine whether immune enhancement can be optimized by delivery in oligosaccharide-enriched low-fat milk.
A double-blind, three-stage before-and-after intervention trial.
Taipei Medical College Hospital, Taipei, Taiwan.
Fifty healthy Taiwanese citizens (age range 41-81; median 60) randomly allocated to two groups.
In stage 1 (run-in control stage) all subjects consumed reconstituted low-fat milk (LFM) for 3 weeks; in stage 2 (probiotic intervention) subjects consumed B. lactis in LFM (group A) or B. lactis in lactose-hydrolysed LFM (group B) for 3 weeks; in stage 3 all subjects returned to non-supplemented LFM for a further 3 weeks (washout stage). The innate immune functions of two different leucocyte types (polymorphonuclear (PMN) cells and natural killer (NK) cells) were assessed at four time points via in vitro analyses on peripheral blood samples.
While consumption of LFM alone had no significant effect on immune responses, stage 2 results indicated significantly enhanced PMN cell phagocytosis and NK cell tumour killing activity following consumption of milk containing B. lactis. These increases levelled off following cessation of B. lactis consumption, but remained above the pre-treatment values. Increases in PMN and NK cell activity were greatest among subjects who consumed B. lactis in lactose-hydrolysed LFM.
Dietary consumption of the probiotic bacterium B. lactis HN019 enhanced immune function of two different types of leucocytes; the degree of enhancement was increased by consuming B. lactis in an oligosaccharide-rich substrate.
Financial support was provided by the New Zealand Dairy Board.
确定一种益生菌乳酸菌菌株（乳酸双歧杆菌HN019）增强免疫的细胞基础；并确定通过富含低聚糖的低脂牛奶递送是否可以优化免疫增强效果。
一项双盲、三阶段前后干预试验。
台湾台北医学院附属医院。
50名健康的台湾公民（年龄范围41 - 81岁；中位数60岁），随机分为两组。
在第1阶段（导入对照阶段），所有受试者饮用复原低脂牛奶（LFM）3周；在第2阶段（益生菌干预阶段），受试者饮用含乳酸双歧杆菌的LFM（A组）或含乳酸双歧杆菌的水解乳糖LFM（B组）3周；在第3阶段，所有受试者再饮用未添加的LFM 3周（洗脱阶段）。通过对外周血样本进行体外分析，在四个时间点评估两种不同白细胞类型（多形核（PMN）细胞和自然杀伤（NK）细胞）的固有免疫功能。
单独饮用LFM对免疫反应无显著影响，而第2阶段的结果表明，饮用含乳酸双歧杆菌的牛奶后，PMN细胞吞噬作用和NK细胞肿瘤杀伤活性显著增强。停止饮用含乳酸双歧杆菌的牛奶后，这些增强作用趋于平稳，但仍高于治疗前水平。在饮用含乳酸双歧杆菌的水解乳糖LFM的受试者中，PMN和NK细胞活性的增加最为显著。
饮食中摄入益生菌乳酸双歧杆菌HN019可增强两种不同类型白细胞的免疫功能；通过在富含低聚糖的底物中摄入乳酸双歧杆菌可增强增强程度。
由新西兰乳业局提供资金支持。
