Yabuuchi E, Kawamura Y, Ezaki T, Ikedo M, Dejsirilert S, Fujiwara N, Naka T, Kobayashi K
Department of Microbiology, Gifu University School of Medicine, Gifu, Japan.
Microbiol Immunol. 2000;44(4):307-17. doi: 10.1111/j.1348-0421.2000.tb02500.x.
A polar multitrichous gram-negative motile rod, EY 3383, originally identified as Burkholderia thailandensis, revealed a DNA-DNA reassociation rate of 36.7%, under stringent conditions, with the type strain of B. thailandensis, despite the 16S rDNA homology value between two type strains being as high as 97.9%. The strain was clearly differentiated from the type strain of B. thailandensis by physiological, bio-chemical, and nutritional characteristics, without significant difference in cellular fatty acid and lipid composition. Based on the results of 16S rDNA sequence analysis, DNA-DNA hybridization and phenotypic characterization, Burkholderia uboniae sp. nov. is herein proposed. The type strain is NCTC 13147=EY 3383, isolated on 8 December 1989 from surface soil along the roadside in Ubon Ratchathani, Thailand. Major respiratory quinone is ubiquinone-8(Q8). G+C content of DNA is 69.71%.
一株极性多毛革兰氏阴性运动杆菌EY 3383，最初鉴定为泰国伯克霍尔德菌，在严格条件下，与泰国伯克霍尔德菌模式菌株的DNA-DNA重结合率为36.7%，尽管两个模式菌株之间的16S rDNA同源性值高达97.9%。该菌株通过生理、生化和营养特征与泰国伯克霍尔德菌模式菌株明显区分，细胞脂肪酸和脂质组成无显著差异。基于16S rDNA序列分析、DNA-DNA杂交和表型特征的结果，在此提出乌汶伯克霍尔德菌新种。模式菌株为NCTC 13147=EY 3383，于1989年12月8日从泰国乌汶叻差他尼路边的表层土壤中分离得到。主要呼吸醌为泛醌-8（Q8）。DNA的G+C含量为69.71%。
