Iuchi S, Easley K, Matsuzaki K, Weiner L, O'Connor N, Green H
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Exp Dermatol. 2000 Jun;9(3):178-84. doi: 10.1034/j.1600-0625.2000.009003178.x.
Basonuclin is a zinc finger protein present in the basal cell layer of the epidermis and in hair follicles. Human basal epidermal cells are often heterogeneous with respect to a nuclear or cytoplasmic location of basonuclin and the protein may be concentrated in either compartment. In mouse and rat epidermis, although clusters of basonuclin may be seen in some basal cell nuclei, the protein is mainly concentrated in the cytoplasm. When epidermis whose basal cells contain predominantly cytoplasmic basonuclin is disaggregated and the cells are cultivated in the presence of supporting 3T3 cells, the basonuclin of the growing keratinocyte colonies is strongly concentrated in the cell nuclei. Transfer of the cells to culture medium without supporting 3T3 cells results in a predominantly cytoplasmic concentration of the basonuclin. This translocation is reversible, since addition of supporting 3T3 cells restores most basonuclin to the nucleus. The nuclear location is associated with more rapid cell growth. We conclude that different states of the keratinocyte require greater or less activity of basonuclin, and the subcellular location of the protein is probably related to the magnitude of its action on the cells.
毛透明蛋白是一种存在于表皮基细胞层和毛囊中的锌指蛋白。人类基底表皮细胞中毛透明蛋白的核定位或胞质定位往往存在异质性，该蛋白可能集中在其中一个区室中。在小鼠和大鼠的表皮中，虽然在一些基底细胞核中可见毛透明蛋白簇，但该蛋白主要集中在细胞质中。当基底细胞主要含有胞质毛透明蛋白的表皮被解离，且细胞在支持性3T3细胞存在的情况下培养时，生长中的角质形成细胞集落的毛透明蛋白会强烈集中在细胞核中。将细胞转移到没有支持性3T3细胞的培养基中会导致毛透明蛋白主要集中在细胞质中。这种易位是可逆的，因为添加支持性3T3细胞会使大多数毛透明蛋白恢复到细胞核中。核定位与更快的细胞生长相关。我们得出结论，角质形成细胞的不同状态需要毛透明蛋白有或多或少的活性，并且该蛋白的亚细胞定位可能与其对细胞作用的大小有关。
