Tseng H, Green H
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115.
J Cell Biol. 1994 Jul;126(2):495-506. doi: 10.1083/jcb.126.2.495.
Basonuclin is a protein possessing three pairs of zinc fingers and a nuclear localization signal. Expression of the gene is largely confined to keratinocytes of stratified squamous epithelia and hair follicles. In the epidermis and in stratified epidermal cultures, basonuclin is present in the nuclei of cells in or close to the basal layer but not in the nuclei of cells in more superficial layers. The Ki-67 protein, a nuclear marker for any stage of the multiplication cycle is present in only a subclass of basonuclin-containing cells. In cultured keratinocytes, the disappearance of basonuclin mRNA is associated with loss of colony-forming ability and the appearance of mRNA for involucrin, a protein characteristic of terminal differentiation. In hair follicles, the largest reservoir of basonuclin-containing cells is the outer root sheath, which contains precursors of differentiated cells of the hair shaft and of the epidermis. Basonuclin is not a cell cycle marker but is likely instead to be a regulatory molecular whose presence in the keratinocyte is linked to the maintenance of proliferative capacity and prevention of terminal differentiation.
毛透明蛋白是一种拥有三对锌指结构和一个核定位信号的蛋白质。该基因的表达主要局限于复层鳞状上皮和毛囊的角质形成细胞。在表皮和分层的表皮培养物中，毛透明蛋白存在于基底层或靠近基底层的细胞的细胞核中，但不存在于更表层细胞的细胞核中。Ki-67蛋白是增殖周期任何阶段的核标记物，仅存在于含毛透明蛋白细胞的一个亚类中。在培养的角质形成细胞中，毛透明蛋白mRNA的消失与集落形成能力的丧失以及外皮蛋白（一种终末分化特征性蛋白质）mRNA的出现相关。在毛囊中，含毛透明蛋白细胞的最大储存库是外根鞘，其包含毛干和表皮分化细胞的前体。毛透明蛋白不是细胞周期标记物，而是可能是一种调节分子，其在角质形成细胞中的存在与增殖能力的维持和终末分化的预防有关。
