Valas S, Benoit C, Baudry C, Perrin G, Mamoun R Z
AFSSA-Niort, Laboratoire de Recherches Caprines, F-79012 Niort Cedex, France.
J Virol. 2000 Jul;74(13):6178-85. doi: 10.1128/jvi.74.13.6178-6185.2000.
The complete surface glycoprotein (SU) nucleotide sequences of three French isolates of caprine arthritis-encephalitis virus (CAEV) were determined and compared with those of previously described isolates: three American isolates and one French isolate. Phylogenetic analyses revealed the existence of four distinct and roughly equidistant evolutionary CAEV subtypes. Four conserved and five variable domains were identified in the SU. The fine specificities of antibodies produced against these domains during natural infection were examined using a pepscan analysis. Nine immunogenic segments were delineated throughout the conserved and variable domains of SU, two of them corresponding to conserved immunodominant epitopes. Antigenic determinants which may be involved in the immunopathogenic process induced by CAEV were identified. These results also provide sensitive and specific antigen peptides for the serological detection and differentiation of CAEV and visna/maedi virus infections.
测定了三株法国山羊关节炎-脑炎病毒（CAEV）分离株完整的表面糖蛋白（SU）核苷酸序列，并与先前描述的分离株进行比较：三株美国分离株和一株法国分离株。系统发育分析揭示存在四种不同且进化距离大致相等的CAEV亚型。在SU中鉴定出四个保守结构域和五个可变结构域。使用肽扫描分析检查了自然感染期间针对这些结构域产生的抗体的精细特异性。在SU的保守和可变结构域中划定了九个免疫原性片段，其中两个对应于保守的免疫显性表位。鉴定了可能参与CAEV诱导的免疫致病过程的抗原决定簇。这些结果还为CAEV和维斯纳/梅迪病毒感染的血清学检测和鉴别提供了敏感且特异的抗原肽。
