Valas S, Benoit C, Guionaud C, Perrin G, Mamoun R Z
IFR INSERM/CNRS No. 66, Pathologies infectieuses, Université Victor Segalen, Bordeaux 2, 146 rue Léo Saignat, Bordeaux Cedex, F-33076, France.
Virology. 1997 Oct 27;237(2):307-18. doi: 10.1006/viro.1997.8800.
The full extent of genetic diversity among small ruminant lentiviruses (SRLVs), i.e., caprine arthritis encephalitis viruses (CAEVs) and maedi-visna viruses (MVVs), remains unknown. This is due in part to the fact that few sequences of CAEV are available. To contribute to this knowledge, gag, pol, and env nucleotide sequences from an SRLV named CA680 originating from a goat from western France were determined. This analysis revealed that this virus is closely related to the Cork and 63 CAEV American isolates. Mismatched amino acids between the CA680 virus and prototype CAEVs ranged from 6.7, 0. 7, and 17.5% for gag, pol, and SU sequences, respectively. The differences between the CA680 virus and MVV prototypes ranged from 16.5, 12.5, and 32.3% for the protein sequences, respectively. A screening using a heteroduplex mobility assay (HMA) adapted to SRLVs revealed that 6 of 10 caprine virus field isolates were closely related to CA680, indicating that this latter isolate was a prototype of CAEVs common in the west of France. Phylogenetic trees drawn using CA, RT, or SU sequences of numerous SRLVs and rooted with EIAV sequences revealed that CA680 and CAEV prototypes, all infectious for goat, clustered in one group. From these HMA and phylogenetic analyses, it appears that U.S. and French caprine SRLVs form a clade that had emerged from a much more diverse group containing all SRLVs infectious for sheep. These ovine SRLVs form a more ancient group in which the EIAV is rooted.
小型反刍动物慢病毒（SRLV），即山羊关节炎脑炎病毒（CAEV）和梅迪 - 维斯纳病毒（MVV）之间的遗传多样性全貌仍不清楚。部分原因是CAEV的序列很少。为了增进这方面的了解，我们测定了一株来自法国西部山羊的名为CA680的SRLV的gag、pol和env核苷酸序列。分析表明，该病毒与Cork和63株CAEV美国分离株密切相关。CA680病毒与原型CAEVs之间gag、pol和SU序列的错配氨基酸分别为6.7%、0.7%和17.5%。CA680病毒与MVV原型之间蛋白质序列的差异分别为16.5%、12.5%和32.3%。使用适用于SRLV的异源双链迁移率分析（HMA）进行筛选发现，10株山羊病毒野外分离株中有6株与CA680密切相关，这表明后者是法国西部常见的CAEVs原型。利用众多SRLV的CA、RT或SU序列绘制并以EIAV序列为根的系统发育树表明，所有对山羊有感染性的CA680和CAEV原型聚集在一组。从这些HMA和系统发育分析来看，美国和法国的山羊SRLV形成了一个进化枝，它从一个包含所有对绵羊有感染性的SRLV的更加多样化的群体中分化出来。这些绵羊SRLV形成了一个更古老的群体，EIAV在其中为根。
