Fukuda T, Kakihara T, Baba K, Yamaki T, Yamaguchi T, Suzuki T
Second Department of Pathology, Fukushima Medical University School of Medicine, Fukushima, Japan.
Pathol Int. 2000 May;50(5):412-6. doi: 10.1046/j.1440-1827.2000.01066.x.
A case of clear cell sarcoma (CCS) arising in the transverse colon is presented. The tumor consisted of sheets or small nests of epithelioid malignant cells possessing pleomorphic nuclei with one or more prominent nucleoli and ample clear or slightly eosinophilic cytoplasm. Some of the tumor cells contained various amounts of melanin pigments that were confirmed by histochemical and ultrastructural examinations. Immunohistochemical examination showed a positive immunoreactivity for HMB45 and S-100 protein. A metastatic nodule, which was found 9 months after surgery, showed similar histological findings to those of the primary one but lacked melanin pigments. Reverse transcriptase- polymerase chain reaction using total ribonucleic acid obtained from metastatic nodule demonstrated the presence of EWS-ATF-1 fusion gene. Based on these findings, the present case tumor is a CCS of the colon.
本文报告一例发生于横结肠的透明细胞肉瘤（CCS）。肿瘤由片状或小巢状上皮样恶性细胞组成，这些细胞具有多形性核，有一个或多个明显的核仁，胞质丰富、清亮或略嗜酸性。部分肿瘤细胞含有不同量的黑色素，经组织化学和超微结构检查得以证实。免疫组化检查显示HMB45和S-100蛋白免疫反应阳性。术后9个月发现的一个转移结节，其组织学表现与原发肿瘤相似，但缺乏黑色素。对从转移结节获取的总核糖核酸进行逆转录聚合酶链反应，证实存在EWS-ATF-1融合基因。基于这些发现，本例肿瘤为结肠CCS。
