Kuhlein E, Laurent G, Rigal F, Delsol G, Pris J, Ducos J
Clin Exp Immunol. 1982 Feb;47(2):389-95.
Mouse erythrocyte-rosette-forming cells (MRFC) were determined in 76 cases of chronic lymphocytic leukaemia (CLL), 19 cases of centroblastic-centrocytic follicular non-Hodgkin's lymphoma (NHL), five cases of hairy cell leukaemia (HCL) and in 62 control patients. The mean percentage of MRFC in B-CLL was 54.8% +/- 24.4%, in nodular centro-follicular NHL 2.3% +/- 3%, in hairy cell leukaemia 14.6% +/-14.7% and among the controls 3.2% +/- 1.9%. Thus, MRFC appear to be an excellent marker of B-CLL and sometimes the only B cell marker as in 11 cases of SIg negative CLL. There is no correlation between the percentage of MRFC and clinical staging according to Rai classification whereas patients with stage 2 in Binet's classification (isolated splenomegaly) have a significantly lower MRFC percentage (23.4% +/- 27.7%; P less than 0.05). There is no correlation with prognosis, nor with serum Ig levels. We found no correlation with surface phenotype IgM, IgM-IgD or IgG. Thus, this study does not confirm the hypothesis that MRFC might characterize and immature stage in the B lymphocyte differentiation. However, these results are consistent with another hypothesis which states that lymphocytes in CLL possibly originate from a clonal expansion of a normal B lymphocyte subset, characterized by a low concentration of SIg and a receptor for mouse erythrocytes.
对76例慢性淋巴细胞白血病（CLL）、19例中心母细胞 - 中心细胞滤泡性非霍奇金淋巴瘤（NHL）、5例毛细胞白血病（HCL）患者以及62例对照患者测定了小鼠红细胞花环形成细胞（MRFC）。B - CLL中MRFC的平均百分比为54.8%±24.4%，结节性中心滤泡性NHL中为2.3%±3%，毛细胞白血病中为14.6%±14.7%，对照组中为3.2%±1.9%。因此，MRFC似乎是B - CLL的一个极佳标志物，在某些情况下，如11例SIg阴性CLL中，它是唯一的B细胞标志物。MRFC百分比与Rai分类的临床分期之间无相关性，而在Binet分类的2期患者（单纯脾肿大）中，MRFC百分比显著较低（23.4%±27.7%；P<0.05）。与预后及血清Ig水平均无相关性。我们未发现与表面表型IgM、IgM - IgD或IgG有相关性。因此，本研究未证实MRFC可能表征B淋巴细胞分化中一个未成熟阶段的假说。然而，这些结果与另一个假说一致，该假说认为CLL中的淋巴细胞可能起源于正常B淋巴细胞亚群的克隆性扩增，其特征为SIg浓度低以及具有小鼠红细胞受体。
