Bentwich Z, Douglas S D, Skutelsky E, Kunkel H G
J Exp Med. 1973 Jun 1;137(6):1532-7. doi: 10.1084/jem.137.6.1532.
Neuraminidase treatment of normal human lymphocytes enhances their capacity to form SRBC rosettes; more red cells are bound and the rosettes are more stable. Under these conditions approximately 90% of peripheral blood lymphocytes form rosettes. In addition to the effects on T cells, another population of lymphocytes which possess surface immunoglobulin and have the Fc receptor acquire the rosette-forming property after neuraminidase. This subpopulation of 'B' cells represents approximately half of the lymphocytes with surface immunoglobulin but is not found among the leukemic lymphocytes of patients with chronic lymphocytic leukemia. Electron microscope observations indicate close approximation and intimate association of the red cell and lymphocyte membranes after neuraminidase.
用神经氨酸酶处理正常人淋巴细胞可增强其形成绵羊红细胞玫瑰花结的能力；结合的红细胞更多，且玫瑰花结更稳定。在这些条件下，约90%的外周血淋巴细胞可形成玫瑰花结。除了对T细胞的作用外，另一群具有表面免疫球蛋白且有Fc受体的淋巴细胞在经神经氨酸酶处理后也获得了形成玫瑰花结的特性。这群“B”细胞亚群约占具有表面免疫球蛋白的淋巴细胞的一半，但在慢性淋巴细胞白血病患者的白血病淋巴细胞中未发现。电子显微镜观察表明，经神经氨酸酶处理后，红细胞膜与淋巴细胞膜紧密靠近并密切结合。
