Gopal R, Gibbs T, Slomka M J, Whitworth J, Carpenter L M, Vyse A, Brown D W
Enteric and Respiratory Virus Laboratory Virus, Central Public Health Laboratory, London, UK.
J Virol Methods. 2000 Jun;87(1-2):71-80. doi: 10.1016/s0166-0934(00)00149-x.
A competitive type-specific enzyme-linked immunosorbent assay (ELISA) for herpes simplex virus type 2 (HSV-2) antibody was developed using an infected cell antigen and a monoclonal antibody to glycoprotein G-2. This assay has been validated for use for epidemiological studies using a large panel of sera collected in rural Uganda and a panel of 143 sera characterised previously by Western blotting, the 'gold standard' for HSV type-specific serology. This evaluation was found to have a sensitivity of 96% and a specificity of 91% in comparison with Western blot on 143 sera from clinic patients. The ELISA had a sensitivity of 93% and a specificity of 91% in comparison with Western blot on 495 sera collected in Uganda. The assay showed good reproducibility and a low percentage of sera gave equivocal results, indicating its suitability for epidemiological studies.
利用感染细胞抗原和针对糖蛋白G-2的单克隆抗体，开发了一种用于检测2型单纯疱疹病毒（HSV-2）抗体的竞争性型特异性酶联免疫吸附测定（ELISA）。该测定方法已通过使用在乌干达农村地区收集的大量血清以及一组先前通过蛋白质印迹法（HSV型特异性血清学的“金标准”）进行鉴定的143份血清，被验证可用于流行病学研究。与来自临床患者的143份血清的蛋白质印迹法相比，该评估的灵敏度为96%，特异性为91%。与在乌干达收集的495份血清的蛋白质印迹法相比，ELISA的灵敏度为93%，特异性为91%。该测定方法显示出良好的重复性，并且给出不确定结果的血清百分比很低，表明其适用于流行病学研究。
