Wales S Q, Smith C C, Wachsman M, Calton G, Aurelian L
AuRx, Inc., Glen Burnie, Maryland 21061, USA.
Clin Diagn Lab Immunol. 2004 Jan;11(1):42-9. doi: 10.1128/cdli.11.1.42-49.2004.
In response to the increasingly evident need for herpes simplex virus (HSV) serotype-specific serologic assays that rely on proteins other than glycoprotein-G (gG), we developed a rapid serologic assay that is based on type-specific epitopes within the large subunit of HSV ribonucleotide reductase (R1). The assay (Au-2 enzyme-linked immunosorbent assay [ELISA]) uses an HSV type 2 (HSV-2) R1 peptide antigen. It provides a reliable method for detecting serotype-specific antibody to a protein other than gG-2. The Au-2 ELISA has high sensitivity and specificity as determined by direct comparison to Western blotting, a widely accepted "gold standard," and to ELISA with an HSV-1 R1 peptide (Au-1). The use of the Au-2 ELISA in conjunction with the gG-2-based assays will improve the sensitivity and specificity of serologic diagnosis and patient management.
鉴于对单纯疱疹病毒（HSV）血清型特异性血清学检测方法的需求日益明显，这种检测方法依赖于糖蛋白-G（gG）以外的蛋白质，我们开发了一种基于HSV核糖核苷酸还原酶大亚基（R1）内型特异性表位的快速血清学检测方法。该检测方法（Au-2酶联免疫吸附测定法[ELISA]）使用HSV 2型（HSV-2）R1肽抗原。它为检测针对gG-2以外蛋白质的血清型特异性抗体提供了一种可靠的方法。通过与广泛认可的“金标准”蛋白质印迹法以及使用HSV-1 R1肽的ELISA（Au-1）直接比较，确定Au-2 ELISA具有高灵敏度和特异性。将Au-2 ELISA与基于gG-2的检测方法结合使用，将提高血清学诊断和患者管理的灵敏度和特异性。
