Romano T J, Mond J J, Thorbecke G J
Eur J Immunol. 1975 Mar;5(3):211-5. doi: 10.1002/eji.1830050312.
Spleens from LAF1 mice injected intravenously with sheep erythrocytes (SE) are relatively rich in memory T cells early in the immune response (1 to 3 days) and rich in memory B cells as the response progresses (2 weeks or more). Marked cooperation for the secondary immune response in vitro was obtained by combining 10(6) spleen cells from LAF1 mice, taken 2 days after intravenous priming with SE, with 10(7) spleen cells from day 14 primed mice. The results indicate relative deficiencies in the spleen for B memory cells on days 1 to 2 and for T memory cells on day 14 after priming. Day -14, but not day -2, immune lymph node (LN) cells could replace the day -2 spleen cells (anti-Thy 1.2 sensitive) in the in vitro cooperation with day -14 immune spleen cells. Immune spleen cells taken 4 to 7 days after priming contain more equivalent numbers of B and T memory cells, but 10 to 7 days after transfer of such immune spleen cells without SE into irradiated recipients the T memory cells were again more prominent in lymph node and the B memory cells in spleen as shown by in vitro cooperation studies. These results suggest that during the second week after intravenous injection of SE relatively more T than B memory cells migrate from spleen to lymph node, resulting in an imbalance in the splenic memory cell population favoring B memory cell function.
静脉注射绵羊红细胞（SE）的LAF1小鼠脾脏在免疫反应早期（1至3天）相对富含记忆T细胞，随着反应进展（2周或更长时间）则富含记忆B细胞。通过将静脉注射SE后2天取自LAF1小鼠的10⁶个脾细胞与第14天致敏小鼠的10⁷个脾细胞相结合，在体外获得了对二次免疫反应的显著协同作用。结果表明，在致敏后第1至2天，脾脏中B记忆细胞相对不足，在第14天T记忆细胞相对不足。第14天而非第2天的免疫淋巴结（LN）细胞可以在与第14天免疫脾细胞的体外协同作用中替代第2天的脾细胞（抗Thy 1.2敏感）。致敏后4至7天获取的免疫脾细胞含有数量大致相等的B和T记忆细胞，但在将此类不含SE的免疫脾细胞转移至受辐照受体后10至7天，体外协同作用研究表明，T记忆细胞在淋巴结中再次更为突出，而B记忆细胞在脾脏中更为突出。这些结果表明，在静脉注射SE后的第二周，相对更多的T记忆细胞而非B记忆细胞从脾脏迁移至淋巴结，导致脾记忆细胞群体失衡，有利于B记忆细胞功能。
