Seymour G J, Dockrell H M, Greenspan J S
Clin Exp Immunol. 1978 Apr;32(1):169-78.
Five enzymes have been studied to differentiate between T and B lymphocytes in sections of human lymph nodes, tonsils and chronic inflammatory periodontal disease. The presence of acid phosphatase, beta-glucuronidase N-acetyl-beta-glucosaminidase, non-specific esterase and fluoride-resistant esterase activity was determined histochemically. The results indicate that cells in the B area of both lymph nodes and tonsils are negative for enzyme activity, while those in the T area show a single intense granule of activity. These enzymes were unable to differentiate between T blasts, B blasts and plasma cells in the sections studied. The majority of the lymphoid cells in the lesions of chronic inflammatory periodontal disease are enzyme-negative and probably of B-cell origin.
为区分人类淋巴结、扁桃体及慢性炎症性牙周病切片中的T淋巴细胞和B淋巴细胞，对五种酶进行了研究。通过组织化学方法测定了酸性磷酸酶、β-葡萄糖醛酸酶、N-乙酰-β-氨基葡萄糖苷酶、非特异性酯酶和耐氟酯酶活性的存在情况。结果表明，淋巴结和扁桃体B区的细胞酶活性均为阴性，而T区的细胞则显示单个强烈的活性颗粒。在所研究的切片中，这些酶无法区分T母细胞、B母细胞和浆细胞。慢性炎症性牙周病病变中的大多数淋巴细胞酶呈阴性，可能起源于B细胞。
