Erb P, Feldmann M, Hogg N
Eur J Immunol. 1976 May;6(5):365-72. doi: 10.1002/eji.1830060512.
Macrophages incubated with soluble antigens for 1-4 days release factors capable of inducing T helper cells. The nature and some of the properties of this genetically related macrophage factor (GRF) were investigated. By use of immunoadsorbents, GRF was shown to contain I region-coded products (Ia antigens) linked to a small antigenic fragment. The genes coding for the Ia antigens detectable in GRF lie in the I region of the H-2 complex. GRF does not react with anti-immunoglobulin, anti-C3 or anti-human beta2-microglobulin antisera, and its activity cannot be removed by antigen immunoadsorbents. GRF is heat-labile, sensitive to proteolytic enzymes and has a molecular weight of about 55 000 Daltons. The relationship of GRF to other factors known to contain I region-coded products and reported to be effective in cell cooperation is discussed.
与可溶性抗原一起孵育1至4天的巨噬细胞会释放能够诱导T辅助细胞的因子。对这种基因相关巨噬细胞因子（GRF）的性质及一些特性进行了研究。通过使用免疫吸附剂，发现GRF含有与一个小抗原片段相连的I区编码产物（Ia抗原）。在GRF中可检测到的Ia抗原的编码基因位于H-2复合体的I区。GRF不与抗免疫球蛋白、抗C3或抗人β2-微球蛋白抗血清发生反应，其活性也不能被抗原免疫吸附剂去除。GRF对热不稳定，对蛋白水解酶敏感，分子量约为55000道尔顿。文中还讨论了GRF与其他已知含有I区编码产物且据报道在细胞协作中有效的因子之间的关系。
