Erb P, Meier B, Feldmann M
J Immunol. 1979 May;122(5):1916-9.
The possibility that the antigen-presenting "macrophages" interacting with helper cells either directly or via the intermediary action of a soluble factor consisting of Ia antigen and a fragment of immunogen, termed GRG (genetically related factor), are a site of Ir gene action was investigated by using the synthetic polypeptide antigen (T,G)-A--L. It was found that T cells from (responder x nonresponder) F1 mice were stimulated by responder "macrophages" or GRF derived from these cells but not by the nonresponder macrophages of GRF from these cells. This suggests that the defect in helper cell induction in nonresponders is at the level of the presenting cell and that the macrophage factor GRF is a soluble Ir gene product. This conclusion was supported by the observation that there was normal presenting cell and GRF function in nonresponders, mouse strains such as CBA that yield helper cells and helper factor with (T,G)-A--L and have defects elsehwere.
通过使用合成多肽抗原（T，G）-A--L，研究了抗原呈递“巨噬细胞”与辅助细胞直接相互作用或通过由Ia抗原和免疫原片段组成的可溶性因子（称为GRG，基因相关因子）的中介作用而成为Ir基因作用位点的可能性。发现来自（应答者×无应答者）F1小鼠的T细胞受到应答者“巨噬细胞”或源自这些细胞的GRF的刺激，但不受这些细胞的无应答者巨噬细胞或GRF的刺激。这表明无应答者中辅助细胞诱导的缺陷在于呈递细胞水平，并且巨噬细胞因子GRF是一种可溶性Ir基因产物。这一结论得到以下观察结果的支持：在无应答者、诸如CBA等小鼠品系中，存在正常的呈递细胞和GRF功能，这些品系能够产生辅助细胞和辅助因子，对（T，G）-A--L有反应，但在其他方面存在缺陷。
