Hirata K, Ishida T, Matsushita H, Tsao P S, Quertermous T
First Department of Internal Medicine, Kobe University School of Medicine, Japan.
Biochem Biophys Res Commun. 2000 May 27;272(1):90-3. doi: 10.1006/bbrc.2000.2747.
A lipoprotein lipase-like gene was recently cloned from endothelial cells. In vitro functional experiments have suggested that this endothelial-derived lipase (EDL) has phospholipase activity, and preliminary in vivo studies have suggested a role in the regulation of high-density lipoprotein metabolism. To investigate local control of lipase activity and lipid metabolism in the blood vessel wall, we have examined the regulation of EDL expression in cultured human umbilical vein and coronary artery endothelial cells. EDL mRNA levels were upregulated in both cell types by inflammatory cytokines implicated in vascular disease etiology, including TNF-alpha and IL-1beta. In addition, both fluid shear stress and cyclic stretch were found to increase the EDL mRNA levels in these cultured cells. This highly regulated expression of EDL in vascular endothelial cells suggests that this recently identified lipase is intricately involved in modulating vessel wall lipid metabolism and may play a role in vascular diseases such as atherosclerosis.
最近从内皮细胞中克隆出一种脂蛋白脂肪酶样基因。体外功能实验表明，这种内皮源性脂肪酶（EDL）具有磷脂酶活性，初步的体内研究表明其在高密度脂蛋白代谢调节中发挥作用。为了研究血管壁中脂肪酶活性和脂质代谢的局部控制，我们检测了培养的人脐静脉和冠状动脉内皮细胞中EDL表达的调节情况。在这两种细胞类型中，与血管疾病病因相关的炎性细胞因子，包括肿瘤坏死因子-α和白细胞介素-1β，均可上调EDL mRNA水平。此外，发现流体剪切应力和周期性拉伸均可增加这些培养细胞中的EDL mRNA水平。EDL在血管内皮细胞中的这种高度调节表达表明，这种最近鉴定出的脂肪酶复杂地参与调节血管壁脂质代谢，并可能在动脉粥样硬化等血管疾病中发挥作用。
