尤因埃立克体和鼠埃立克体groESL操纵子序列的PCR扩增及系统发育分析
PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris.
作者信息
Sumner J W, Storch G A, Buller R S, Liddell A M, Stockham S L, Rikihisa Y, Messenger S, Paddock C D
机构信息
Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 2000 Jul;38(7):2746-9. doi: 10.1128/JCM.38.7.2746-2749.2000.
Abstract
Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.
摘要
广谱聚合酶链反应（PCR）引物被用于扩增犬病原体尤因埃立克体（Ehrlichia ewingii，最近被确认为一种人类病原体）以及鼠病原体鼠埃立克体（Ehrlichia muris）的groESL操纵子的部分序列。系统发育分析支持了先前通过16S rRNA基因序列比较所确定的埃立克体物种之间的关系。这些序列为那些已确定基因序列很少的物种提供了额外的PCR靶点。
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