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1
PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris.尤因埃立克体和鼠埃立克体groESL操纵子序列的PCR扩增及系统发育分析
J Clin Microbiol. 2000 Jul;38(7):2746-9. doi: 10.1128/JCM.38.7.2746-2749.2000.
2
[Study of the heterogeneity of 16s rRNA gene and groESL operone in the dna samples of Anaplasma phagocytophilum, Ehrlichia muris, and "Candidatus Neoehrlichia mikurensis" determined in the Ixodes persulcatus ticks in the area of Urals, Siberia, and far east of Russia].[对俄罗斯乌拉尔地区、西伯利亚和远东地区全沟硬蜱中嗜吞噬细胞无形体、鼠埃立克体及“新埃立克体候选种”DNA样本中16S rRNA基因和groESL操纵子异质性的研究]
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3
Comparison of the nucleotide sequences of 16S rRNA, 444 Ep-ank, and groESL heat shock operon genes in naturally occurring Ehrlichia equi and human granulocytic ehrlichiosis agent isolates from Northern California.来自北加利福尼亚州自然存在的马埃立克体和人粒细胞埃立克体病病原体分离株中16S rRNA、444 Ep-ank和groESL热休克操纵子基因核苷酸序列的比较。
J Clin Microbiol. 2000 Apr;38(4):1364-9. doi: 10.1128/JCM.38.4.1364-1369.2000.
4
PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species.埃立克体属groESL热休克操纵子的PCR扩增及核苷酸序列比较
J Clin Microbiol. 1997 Aug;35(8):2087-92. doi: 10.1128/jcm.35.8.2087-2092.1997.
5
Determination of the nucleotide sequences of heat shock operon groESL and the citrate synthase gene (gltA) of Anaplasma (Ehrlichia) platys for phylogenetic and diagnostic studies.测定嗜吞噬细胞无形体（埃立克体）血小板亚种热休克操纵子groESL和柠檬酸合酶基因（gltA）的核苷酸序列用于系统发育和诊断研究。
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Coinfection with three Ehrlichia species in dogs from Thailand and Venezuela with emphasis on consideration of 16S ribosomal DNA secondary structure.泰国和委内瑞拉犬类感染三种埃立克体的混合感染，重点关注16S核糖体DNA二级结构的考量
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Identification of a p28 gene in Ehrlichia ewingii: evaluation of gene for use as a target for a species-specific PCR diagnostic assay.尤因埃立克体中p28基因的鉴定：对用作种特异性PCR诊断检测靶标的基因进行评估。
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Predominance of Ehrlichia ewingii in Missouri dogs.埃氏尤因埃立克体在密苏里州犬类中的优势地位。
J Clin Microbiol. 2003 Oct;41(10):4617-22. doi: 10.1128/JCM.41.10.4617-4622.2003.
9
Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila.立克次氏体目里克次体科和无形体科属的重新分类：将埃立克体属的一些物种与无形体属合并、考德里体属与埃立克体属合并以及埃立克体属与新立克次体属合并，六个新物种组合的描述以及将马埃立克体和“人粒细胞埃立克体病病原体”指定为嗜吞噬细胞埃立克体的主观同义词。
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Ehrlichia ewingii sp. nov., the etiologic agent of canine granulocytic ehrlichiosis.尤因埃立克体新种，犬粒细胞埃立克体病的病原体。
Int J Syst Bacteriol. 1992 Apr;42(2):299-302. doi: 10.1099/00207713-42-2-299.

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MyD88-dependent inflammasome activation and autophagy inhibition contributes to Ehrlichia-induced liver injury and toxic shock.依赖髓样分化因子88的炎性小体激活和自噬抑制促成了埃立克体诱导的肝损伤和中毒性休克。
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Development of a generic Ehrlichia FRET-qPCR and investigation of ehrlichioses in domestic ruminants on five Caribbean islands.一种通用埃立克体荧光共振能量转移定量聚合酶链反应的开发以及对五个加勒比岛屿上家养反刍动物埃立克体病的调查。
Parasit Vectors. 2015 Oct 6;8:506. doi: 10.1186/s13071-015-1118-5.
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Identification of 19 polymorphic major outer membrane protein genes and their immunogenic peptides in Ehrlichia ewingii for use in a serodiagnostic assay.尤因埃立克体中19个多态性主要外膜蛋白基因及其免疫原性肽的鉴定，用于血清诊断检测。
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Infect Immun. 2007 Sep;75(9):4608-20. doi: 10.1128/IAI.00198-07. Epub 2007 Jun 11.
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Role of tumor necrosis factor alpha (TNF-alpha) and interleukin-10 in the pathogenesis of severe murine monocytotropic ehrlichiosis: increased resistance of TNF receptor p55- and p75-deficient mice to fatal ehrlichial infection.肿瘤坏死因子α（TNF-α）和白细胞介素-10在严重鼠单核细胞埃立克体病发病机制中的作用：TNF受体p55和p75缺陷小鼠对致命埃立克体感染的抵抗力增强
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Determination of the nucleotide sequences of heat shock operon groESL and the citrate synthase gene (gltA) of Anaplasma (Ehrlichia) platys for phylogenetic and diagnostic studies.测定嗜吞噬细胞无形体（埃立克体）血小板亚种热休克操纵子groESL和柠檬酸合酶基因（gltA）的核苷酸序列用于系统发育和诊断研究。
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10
Identification of a p28 gene in Ehrlichia ewingii: evaluation of gene for use as a target for a species-specific PCR diagnostic assay.尤因埃立克体中p28基因的鉴定：对用作种特异性PCR诊断检测靶标的基因进行评估。
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本文引用的文献

1
Dusky-footed wood rats (Neotoma fuscipes) as reservoirs of granulocytic Ehrlichiae (Rickettsiales: Ehrlichieae) in northern California.作为加利福尼亚州北部粒细胞埃立克体（立克次氏体目：埃立克体科）宿主的暗足林鼠（Neotoma fuscipes）
J Clin Microbiol. 1999 Oct;37(10):3323-7. doi: 10.1128/JCM.37.10.3323-3327.1999.
2
Coinfection with multiple tick-borne pathogens in a Walker Hound kennel in North Carolina.北卡罗来纳州一只沃克猎犬犬舍中多种蜱传病原体的混合感染。
J Clin Microbiol. 1999 Aug;37(8):2631-8. doi: 10.1128/JCM.37.8.2631-2638.1999.
3
Ehrlichia ewingii, a newly recognized agent of human ehrlichiosis.尤因埃立克体，一种新确认的人类埃立克体病病原体。
N Engl J Med. 1999 Jul 15;341(3):148-55. doi: 10.1056/NEJM199907153410303.
4
Comparison of Ehrlichia muris strains isolated from wild mice and ticks and serologic survey of humans and animals with E. muris as antigen.从野生小鼠和蜱虫中分离出的鼠埃立克体菌株的比较以及以鼠埃立克体为抗原对人和动物进行的血清学调查。
J Clin Microbiol. 1999 Apr;37(4):1123-9. doi: 10.1128/JCM.37.4.1123-1129.1999.
5
Serologic testing for human granulocytic ehrlichiosis at a national referral center.在一家国家级转诊中心进行的人粒细胞埃立克体病血清学检测。
J Clin Microbiol. 1999 Mar;37(3):558-64. doi: 10.1128/JCM.37.3.558-564.1999.
6
Identity of ehrlichial DNA sequences derived from Ixodes ricinus ticks with those obtained from patients with human granulocytic ehrlichiosis in Slovenia.源自蓖麻硬蜱的埃立克体DNA序列与斯洛文尼亚人类粒细胞埃立克体病患者体内获得的序列的一致性。
J Clin Microbiol. 1999 Jan;37(1):209-10. doi: 10.1128/JCM.37.1.209-210.1999.
7
A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma.对来自俄克拉荷马州的犬类和蜱虫进行的犬埃立克体、查菲埃立克体和尤因埃立克体的分子与血清学调查。
Vet Parasitol. 1998 Nov 27;79(4):325-39. doi: 10.1016/s0304-4017(98)00179-4.
8
Human granulocytic ehrlichiosis in Europe: clinical and laboratory findings for four patients from Slovenia.欧洲的人粒细胞埃立克体病：来自斯洛文尼亚的4例患者的临床和实验室检查结果
Clin Infect Dis. 1998 Sep;27(3):424-8. doi: 10.1086/514683.
9
Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii, or Bartonella vinsonii.对自然感染犬埃立克体、查菲埃立克体、马埃立克体、尤因埃立克体或文森巴尔通体的犬进行序贯评估。
J Clin Microbiol. 1998 Sep;36(9):2645-51. doi: 10.1128/JCM.36.9.2645-2651.1998.
10
Granulocytic ehrlichiosis in dogs from North Carolina and Virginia.北卡罗来纳州和弗吉尼亚州犬类的粒细胞埃立克体病
J Vet Intern Med. 1998 Mar-Apr;12(2):61-70. doi: 10.1111/j.1939-1676.1998.tb02096.x.

尤因埃立克体和鼠埃立克体groESL操纵子序列的PCR扩增及系统发育分析

PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris.

作者信息

Sumner J W, Storch G A, Buller R S, Liddell A M, Stockham S L, Rikihisa Y, Messenger S, Paddock C D

机构信息

Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

出版信息

J Clin Microbiol. 2000 Jul;38(7):2746-9. doi: 10.1128/JCM.38.7.2746-2749.2000.

DOI:10.1128/JCM.38.7.2746-2749.2000
PMID:10878077
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC87017/
Abstract

Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.

摘要

广谱聚合酶链反应（PCR）引物被用于扩增犬病原体尤因埃立克体（Ehrlichia ewingii，最近被确认为一种人类病原体）以及鼠病原体鼠埃立克体（Ehrlichia muris）的groESL操纵子的部分序列。系统发育分析支持了先前通过16S rRNA基因序列比较所确定的埃立克体物种之间的关系。这些序列为那些已确定基因序列很少的物种提供了额外的PCR靶点。