源自蓖麻硬蜱的埃立克体DNA序列与斯洛文尼亚人类粒细胞埃立克体病患者体内获得的序列的一致性。
Identity of ehrlichial DNA sequences derived from Ixodes ricinus ticks with those obtained from patients with human granulocytic ehrlichiosis in Slovenia.
作者信息
Petrovec M, Sumner J W, Nicholson W L, Childs J E, Strle F, Barlic J, Lotric-Furlan S, Avsic Zupanc T
机构信息
Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia.
出版信息
J Clin Microbiol. 1999 Jan;37(1):209-10. doi: 10.1128/JCM.37.1.209-210.1999.
Abstract
Adult Ixodes ricinus (Acari: Ixodidae) ticks collected near Ljubljana, Slovenia, were tested for the agent of human granulocytic ehrlichiosis (HGE) by using PCR assays based on the 16S rRNA gene. Three (3.2%) of 93 ticks were found to contain granulocytic ehrlichiae. Nucleotide sequences of portions of the bacterial groESL heat shock operon amplified from these ticks were identical or nearly (99.8%) identical to those previously determined for human patients with HGE from Slovenia, providing additional evidence that the ticks were infected with the HGE agent. This study identified I. ricinus as the likely vector for these ehrlichial pathogens of humans in this part of Europe.
摘要
采用基于16S rRNA基因的聚合酶链反应（PCR）检测法，对在斯洛文尼亚卢布尔雅那附近采集的成年蓖麻硬蜱（蜱螨亚纲：硬蜱科）进行人粒细胞埃立克体病（HGE）病原体检测。在93只蜱中，有3只（3.2%）被发现含有粒细胞埃立克体。从这些蜱中扩增出的细菌groESL热休克操纵子部分的核苷酸序列，与先前从斯洛文尼亚HGE患者中测定的序列相同或几乎相同（99.8%），这进一步证明这些蜱感染了HGE病原体。本研究确定蓖麻硬蜱可能是欧洲该地区这些人类埃立克体病原体的传播媒介。
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