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Identity of ehrlichial DNA sequences derived from Ixodes ricinus ticks with those obtained from patients with human granulocytic ehrlichiosis in Slovenia.源自蓖麻硬蜱的埃立克体DNA序列与斯洛文尼亚人类粒细胞埃立克体病患者体内获得的序列的一致性。
J Clin Microbiol. 1999 Jan;37(1):209-10. doi: 10.1128/JCM.37.1.209-210.1999.
2
Ehrlichial DNA amplified from Ixodes ricinus (Acari: Ixodidae) in France.在法国从蓖麻硬蜱（蜱螨亚纲：硬蜱科）中扩增出的埃立克体DNA。
J Med Entomol. 1998 Mar;35(2):180-3. doi: 10.1093/jmedent/35.2.180.
3
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4
Detection of HGE agent-like Ehrlichia in Ixodes ricinus ticks in northern Italy by PCR.通过聚合酶链反应（PCR）检测意大利北部蓖麻硬蜱中类嗜吞噬细胞无形体埃立克体
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本文引用的文献

1
The human granulocytic ehrlichiosis (HGE) agent in Swedish ticks.瑞典蜱虫中的人粒细胞埃立克体病（HGE）病原体。
Clin Microbiol Infect. 1997;3(5):573-574. doi: 10.1111/j.1469-0691.1997.tb00311.x.
2
Human granulocytic ehrlichiosis in Europe: clinical and laboratory findings for four patients from Slovenia.欧洲的人粒细胞埃立克体病：来自斯洛文尼亚的4例患者的临床和实验室检查结果
Clin Infect Dis. 1998 Sep;27(3):424-8. doi: 10.1086/514683.
3
Ehrlichial DNA amplified from Ixodes ricinus (Acari: Ixodidae) in France.在法国从蓖麻硬蜱（蜱螨亚纲：硬蜱科）中扩增出的埃立克体DNA。
J Med Entomol. 1998 Mar;35(2):180-3. doi: 10.1093/jmedent/35.2.180.
4
Coexistence of Ehrlichia phagocytophila and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Italy as determined by 16S rRNA gene sequencing.通过16S rRNA基因测序确定意大利蓖麻硬蜱中嗜吞噬细胞无形体与狭义伯氏疏螺旋体的共存情况。
J Clin Microbiol. 1997 Dec;35(12):3365-6. doi: 10.1128/jcm.35.12.3365-3366.1997.
5
PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species.埃立克体属groESL热休克操纵子的PCR扩增及核苷酸序列比较
J Clin Microbiol. 1997 Aug;35(8):2087-92. doi: 10.1128/jcm.35.8.2087-2092.1997.
6
Human disease in Europe caused by a granulocytic Ehrlichia species.欧洲由粒细胞埃立克体物种引起的人类疾病。
J Clin Microbiol. 1997 Jun;35(6):1556-9. doi: 10.1128/jcm.35.6.1556-1559.1997.
7
Infection rate of Ixodes ricinus ticks with Borrelia afzelii, Borrelia garinii, and Borrelia burgdorferi sensu stricto in Slovenia.斯洛文尼亚蓖麻硬蜱感染阿氏疏螺旋体、伽氏疏螺旋体和狭义伯氏疏螺旋体的感染率。
Eur J Clin Microbiol Infect Dis. 1995 Nov;14(11):994-1001. doi: 10.1007/BF01691382.
8
Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences.基于线粒体16S rDNA序列的硬蜱和软蜱类群（蜱螨亚纲：硬蜱目）系统发育
Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):10034-8. doi: 10.1073/pnas.91.21.10034.
9
Ixodes dammini as a potential vector of human granulocytic ehrlichiosis.达氏硬蜱作为人类粒细胞埃立克体病的潜在传播媒介。
J Infect Dis. 1995 Oct;172(4):1007-12. doi: 10.1093/infdis/172.4.1007.
10
Tick-borne fever: a review.蜱传发热：综述
Vet Res Commun. 1983 Jun;6(3):163-75. doi: 10.1007/BF02214910.

源自蓖麻硬蜱的埃立克体DNA序列与斯洛文尼亚人类粒细胞埃立克体病患者体内获得的序列的一致性。

Identity of ehrlichial DNA sequences derived from Ixodes ricinus ticks with those obtained from patients with human granulocytic ehrlichiosis in Slovenia.

作者信息

Petrovec M, Sumner J W, Nicholson W L, Childs J E, Strle F, Barlic J, Lotric-Furlan S, Avsic Zupanc T

机构信息

Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia.

出版信息

J Clin Microbiol. 1999 Jan;37(1):209-10. doi: 10.1128/JCM.37.1.209-210.1999.

DOI:10.1128/JCM.37.1.209-210.1999
PMID:9854093
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC84210/
Abstract

Adult Ixodes ricinus (Acari: Ixodidae) ticks collected near Ljubljana, Slovenia, were tested for the agent of human granulocytic ehrlichiosis (HGE) by using PCR assays based on the 16S rRNA gene. Three (3.2%) of 93 ticks were found to contain granulocytic ehrlichiae. Nucleotide sequences of portions of the bacterial groESL heat shock operon amplified from these ticks were identical or nearly (99.8%) identical to those previously determined for human patients with HGE from Slovenia, providing additional evidence that the ticks were infected with the HGE agent. This study identified I. ricinus as the likely vector for these ehrlichial pathogens of humans in this part of Europe.

摘要

采用基于16S rRNA基因的聚合酶链反应（PCR）检测法，对在斯洛文尼亚卢布尔雅那附近采集的成年蓖麻硬蜱（蜱螨亚纲：硬蜱科）进行人粒细胞埃立克体病（HGE）病原体检测。在93只蜱中，有3只（3.2%）被发现含有粒细胞埃立克体。从这些蜱中扩增出的细菌groESL热休克操纵子部分的核苷酸序列，与先前从斯洛文尼亚HGE患者中测定的序列相同或几乎相同（99.8%），这进一步证明这些蜱感染了HGE病原体。本研究确定蓖麻硬蜱可能是欧洲该地区这些人类埃立克体病原体的传播媒介。