Goldman E E, Breitschwerdt E B, Grindem C B, Hegarty B C, Walls J J, Dumler J S
Department of Microbiology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
J Vet Intern Med. 1998 Mar-Apr;12(2):61-70. doi: 10.1111/j.1939-1676.1998.tb02096.x.
Medical records of 3 dogs from North Carolina and 3 dogs from Virginia with ehrlichial morulae in circulating neutrophils were studied retrospectively. Two clinically distinct disease syndromes, including chronic, moderate to severe anemia (n = 3) and polyarthritis (n = 2) were associated with canine granulocytic ehrlichiosis (CGE) in these dogs. One dog was clinically healthy, and abnormalities were not detected during physical examination. Clinical signs were nonspecific and included fever, lethargy, anorexia, vomiting, and diarrhea. The most frequent laboratory abnormalities were normocytic normochromic nonregenerative anemia, moderate thrombocytopenia with large platelets, lymphopenia, and eosinopenia. Considerable variability was found in the serologic responses to Ehrlichia equi, Ehrlichia canis, and Ehrlichia chaffeensis antigens among the 5 dogs for which stored sera were available for indirect fluorescent antibody testing. Polymerase chain reaction amplification and sequencing of portions of the 16S rRNA gene from blood (collected in ethylenediaminetetraacetic acid) of 1 severely anemic dog (dog 3) and 1 polyarthritic dog (dog 4) resulted in DNA sequences nearly identical to the GenBank accessions for Ehrlichia ewingii. The DNA sequence from a 3rd dog (dog 5) was most similar to that of E. canis. Serologic or molecular results support the possibility of E. ewingii, E. equi, and E. canis coinfection or serologic cross-reactivity among canine granulocytic and monocytic Ehrlichia species in dogs from North Carolina and Virginia. Variability in response to tetracycline or doxycycline treatment was noted in these dogs, with more rapid resolution of signs in dogs with polyarthritis. We report the 1st cases of CGE in dogs from North Carolina and Virginia, including recognition of CGE in a healthy dog.
对来自北卡罗来纳州的3只犬和来自弗吉尼亚州的3只循环嗜中性粒细胞中存在埃立克体桑葚体的犬的病历进行了回顾性研究。在这些犬中，两种临床上不同的疾病综合征，包括慢性、中度至重度贫血（n = 3）和多关节炎（n = 2）与犬粒细胞埃立克体病（CGE）相关。1只犬临床健康，体格检查未发现异常。临床症状无特异性，包括发热、嗜睡、厌食、呕吐和腹泻。最常见的实验室异常为正细胞正色素性非再生性贫血、伴有大血小板的中度血小板减少、淋巴细胞减少和嗜酸性粒细胞减少。在5只可获得储存血清用于间接荧光抗体检测的犬中，发现它们对马埃立克体、犬埃立克体和查菲埃立克体抗原的血清学反应存在相当大的差异。对1只重度贫血犬（犬3）和1只多关节炎犬（犬4）的血液（采集于乙二胺四乙酸）进行16S rRNA基因部分片段的聚合酶链反应扩增和测序，结果得到的DNA序列与埃文斯埃立克体的GenBank登录号几乎相同。第3只犬（犬5）的DNA序列与犬埃立克体的最相似。血清学或分子学结果支持在来自北卡罗来纳州和弗吉尼亚州的犬中，埃文斯埃立克体、马埃立克体和犬埃立克体共感染或犬粒细胞和单核细胞埃立克体物种之间血清学交叉反应的可能性。在这些犬中，注意到对四环素或强力霉素治疗的反应存在差异，多关节炎犬的症状缓解更快。我们报告了北卡罗来纳州和弗吉尼亚州犬中CGE的首例病例，包括在1只健康犬中识别出CGE。
