Tolstrup N, Sensen C W, Garrett R A, Clausen I G
Novo Nordisk A/S, Enzyme Research, Bioinformatics and DNA Sequencing, Bagsvaerd, Denmark.
Extremophiles. 2000 Jun;4(3):175-9. doi: 10.1007/s007920070032.
The translational starts of 144 Sulfolobus solfataricus genes have been determined by database comparison. Half the genes lie inside operons and the other half are at the start of an operon or single genes. A Shine-Dalgarno sequence is found upstream of the genes inside operons, but not for the first gene in an operon or isolated genes; this indicates that two different mechanisms are used for translation initiation in S. solfataricus. A box A transcriptional signal is found for the genes starting an operon or isolated genes, but not for the genes inside an operon. The box A signal is located about 27 nt upstream of the start codon, which implies that little or no upstream sequence is available for translation initiation for this group of genes. This finding is discussed.
通过数据库比较确定了144个嗜热栖热菌基因的翻译起始位点。一半的基因位于操纵子内，另一半位于操纵子的起始处或单个基因处。在操纵子内的基因上游发现了Shine-Dalgarno序列，但在操纵子的第一个基因或孤立基因中未发现；这表明嗜热栖热菌在翻译起始时使用了两种不同的机制。对于起始操纵子或孤立基因的基因发现了盒A转录信号，但对于操纵子内的基因未发现。盒A信号位于起始密码子上游约27个核苷酸处，这意味着这组基因用于翻译起始的上游序列很少或没有。对这一发现进行了讨论。
