Yamakuchi M, Higuchi I, Masuda S, Ohira Y, Kubo T, Kato Y, Maruyama I, Kitajima I
Department of Molecular Laboratory Medicine, Kagoshima University School of Medicine, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.
FEBS Lett. 2000 Jul 14;477(1-2):135-40. doi: 10.1016/s0014-5793(00)01715-4.
To investigate the molecular mechanisms of muscle atrophy under microgravity, the paraspinal muscles of rats after 14 days spaceflight and those of ground-based controls were examined. In the microgravitational environment, expressions of 42 genes changed, and the expressions of heat shock protein 70 and t complex polypeptide 1 increased. In Northern blotting, myocyte-specific enhancer binding factor 2C (MEF2C) and MEF2C-related genes including aldolase A and muscle ankyrin decreased. After 9 days ground recovery, expression of MEF2C increased and it was located mainly on the satellite cells in the muscle regeneration state. MEF2C could be a key transcriptional factor for skeletal muscle atrophy and regeneration under microgravity.
为研究微重力条件下肌肉萎缩的分子机制，对经过14天太空飞行的大鼠椎旁肌以及地面对照组大鼠的椎旁肌进行了检测。在微重力环境中，42个基因的表达发生了变化，热休克蛋白70和t复合体多肽1的表达增加。在Northern印迹法检测中，肌细胞特异性增强子结合因子2C（MEF2C）以及包括醛缩酶A和肌肉锚蛋白在内的MEF2C相关基因表达下降。地面恢复9天后，MEF2C的表达增加，且主要定位于处于肌肉再生状态的卫星细胞上。MEF2C可能是微重力条件下骨骼肌萎缩和再生的关键转录因子。
