Edberg S C, Chu A
Am J Med Technol. 1975 Mar;41(3):99-105.
Methods for the rapid determination of antibiotic blood levels require particular organisms or special conditions or long incubation. A method was developed for the rapid determination of antibiotic blood levels which requires no special equipment and is rapid. Special stains are not required. Utilizing bacteria from standard Kirby-Bauer sensitivity plates, a suspension is prepared and a pour plate made. Organisms which are sensitive to the questioned antibiotic and resistant to other antibiotics the patient is receiving are chosen. Muller-Hinton is the best general agar. Wells are cut, and antibiotic standards, antibiotic controls, and the patient's serum introduced. Within three to four hours, zone sizes can be read and the antibiotic blood level calculated. The materials and technical facility required should not preclude any laboratory from successfully performing the method.
快速测定抗生素血药浓度的方法需要特定的微生物、特殊条件或长时间孵育。现已开发出一种快速测定抗生素血药浓度的方法，该方法无需特殊设备且速度快。不需要特殊染色。利用标准 Kirby-Bauer 药敏平板上的细菌，制备悬浮液并制作倾注平板。选择对所怀疑抗生素敏感且对患者正在使用的其他抗生素耐药的微生物。Muller-Hinton 是最佳的通用琼脂。打孔后，加入抗生素标准品、抗生素对照品和患者血清。三到四个小时内，即可读取抑菌圈大小并计算抗生素血药浓度。所需的材料和技术设备不应妨碍任何实验室成功实施该方法。
