血清庆大霉素酶免疫测定法的评估
Evaluation of an enzyme immunoassay for serum gentamicin.
作者信息
O'Leary T D, Ratcliff R M, Geary T D
出版信息
Antimicrob Agents Chemother. 1980 May;17(5):776-8. doi: 10.1128/AAC.17.5.776.
Abstract
The EMIT (Enzyme Multiplied Immunoassay Technique) for serum gentamicin determination was evaluated by a standard procedure. The precision, accuracy, and specificity were assessed and proved satisfactory. Comparison with a bioassay was done, and results for patient samples showed a correlation coefficient of 0.95 between the two methods. Advantages of the enzyme immunoassay system were the provision of results within 15 min of receipt of blood in the laboratory, a requirement of minimal technical expertise, and an applicability to both large and small workloads. The EMIT proved during evaluation to be a practical alternative to current bioassays for the determination of serum gentamicin concentrations.
摘要
采用标准程序对用于血清庆大霉素测定的EMIT（酶放大免疫测定技术）进行了评估。评估了其精密度、准确度和特异性，结果令人满意。与生物测定法进行了比较，患者样本的检测结果表明两种方法之间的相关系数为0.95。酶免疫测定系统的优点包括在实验室收到血液后15分钟内即可提供结果、对技术专业知识要求极低，以及适用于各种工作量。在评估过程中，EMIT被证明是目前用于测定血清庆大霉素浓度的生物测定法的一种实用替代方法。
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