Lee J K, Kim D B, Kim J I, Kim P Y
Department of Toxicology, National Institute of Toxicology Research, Korea Food and Drug Administration, 122-704, Seoul, South Korea.
Toxicol In Vitro. 2000 Aug;14(4):345-9. doi: 10.1016/s0887-2333(00)00028-x.
Cultured human skin cells are a potentially useful model for skin irritancy testing. We have investigated the use of human skin fibroblasts for in vitro screening for skin toxicity. To assess the cytotoxic effects of surfactants, cell viability was measured by the NRU (neutral red uptake) assay and AB (Alamar blue) assay as in vitro methods. The skin irritation potential of surfactants by human skin patch test was assessed as in vivo methods. The close relationship was found between AB assay with human skin fibroblasts and human patch test (r=0.867). There was a relatively good agreement between the NRU and in vivo patch test (r=0.648). These results suggest that AB and NRU assay using cultured human fibroblast could be predictable methods for the irritancy of various surfactants in human.
培养的人皮肤细胞是皮肤刺激性测试的一种潜在有用模型。我们研究了使用人皮肤成纤维细胞进行皮肤毒性的体外筛选。为了评估表面活性剂的细胞毒性作用，采用体外方法通过NRU（中性红摄取）试验和AB（alamar蓝）试验测量细胞活力。采用体内方法通过人体皮肤斑贴试验评估表面活性剂的皮肤刺激潜力。发现人皮肤成纤维细胞的AB试验与人体斑贴试验之间存在密切关系（r = 0.867）。NRU试验与体内斑贴试验之间存在相对较好的一致性（r = 0.648）。这些结果表明，使用培养的人成纤维细胞进行AB和NRU试验可能是预测各种表面活性剂对人体刺激性的方法。
