Jacobs D M, Morrison D C
J Immunol. 1975 Jan;114(1 Pt 2):360-4.
The trinitrophenyl hapten (TNP) has been covalently conjugated to bacterial lipopolysaccharides (LPS) to give TNP-LPS. The site of attachment has been suggested to be in the core polysaccharide and lipid A region of the molecule and approximately 2.4 hapten molecules are bound per monomer LPS molecule. The TNP-LPS has been demonstrated to be immunogenic in vitro at very low concentration. This antigen has further been shown to initiate a T-independent TNP-PFC response. The immunogenicity of TNP-LPS is abrogated by mild alkaline hydrolysis, suggesting a requirement for intact lipid A in the initiation of an immune respose at the very low concentrations of antigen used.
三硝基苯基半抗原（TNP）已与细菌脂多糖（LPS）共价结合，形成TNP-LPS。据推测，结合位点位于分子的核心多糖和脂质A区域，每个单体LPS分子约结合2.4个半抗原分子。已证明TNP-LPS在极低浓度下在体外具有免疫原性。该抗原还进一步显示可引发非T细胞依赖性的TNP-PFC反应。轻度碱性水解可消除TNP-LPS的免疫原性，这表明在使用极低浓度抗原引发免疫反应时，需要完整的脂质A。
