Taki H, Sugiyama E, Kuroda A, Mino T, Kobayashi M
First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
Rheumatology (Oxford). 2000 Jul;39(7):728-31. doi: 10.1093/rheumatology/39.7.728.
To examine the effect of interleukin-4 (IL-4) on IL-11 production by rheumatoid synovial cells.
Freshly isolated rheumatoid synovial cells (FRS) were obtained by collagenase digestion of rheumatoid arthritis (RA) synovial tissue specimens taken at the time of operation. Rheumatoid synovial cells at four to eight passages were used as cultured rheumatoid synovial fibroblasts (RSF). IL-11 concentration was measured by ELISA.
IL-4 inhibited the production of IL-11 by FRS in a dose-dependent manner. This inhibition was observed in FRS obtained from six patients, and the mean inhibition was 46.5%. The inhibitory effect of IL-4 on IL-11 production was cancelled by the addition of anti-IL-4 antibody. IL-4 also inhibited IL-11 production by IL-1alpha-stimulated cultured RSF.
IL-4 inhibited IL-11 production by rheumatoid synovial cells. IL-4 has a protective effect on bone resorption. On the contrary, IL-11 participates in bone resorption via osteoclastogenesis. Therefore, IL-4 may exert its protective effect on bone resorption, at least in part, via inhibition of IL-11 production in rheumatoid joints.
研究白细胞介素-4（IL-4）对类风湿性滑膜细胞产生IL-11的影响。
通过胶原酶消化手术时获取的类风湿性关节炎（RA）滑膜组织标本，获得新鲜分离的类风湿性滑膜细胞（FRS）。将传代4至8次的类风湿性滑膜细胞用作培养的类风湿性滑膜成纤维细胞（RSF）。采用酶联免疫吸附测定法（ELISA）检测IL-11浓度。
IL-4以剂量依赖性方式抑制FRS产生IL-11。在6例患者的FRS中均观察到这种抑制作用，平均抑制率为46.5%。添加抗IL-4抗体可消除IL-4对IL-11产生的抑制作用。IL-4还抑制IL-1α刺激的培养RSF产生IL-11。
IL-4抑制类风湿性滑膜细胞产生IL-11。IL-4对骨吸收具有保护作用。相反，IL-11通过破骨细胞生成参与骨吸收。因此，IL-4可能至少部分地通过抑制类风湿关节中IL-11的产生，对骨吸收发挥其保护作用。
