Nigam J N
Biochemistry Division, Regional Research Laboratory, Jorhat 785 006, Assam, India.
J Biotechnol. 2000 Jun 23;80(2):189-93. doi: 10.1016/s0168-1656(00)00246-7.
The cells of Saccharomyces cerevisiae ATCC 24553, were immobilized in k-carrageenan and packed in a tapered glass column reactor for ethanol production from pineapple cannery waste at temperature 30 degrees C and pH 4.5. The maximum productivity was 42.8 g ethanol 1(-1) h(-1) at a dilution rate of 1.5 h(-1). The volumetric ethanol productivity of the immobilized cells was ca. 11.5 times higher than the free cells. The immobilized cell reactor was operated over a period of 87 days at a dilution rate of 1.0 h(-1), without any loss in the immobilized cell activity. The maximum specific ethanol productivity and specific sugar uptake rate of the immobilized cells were 1.2 g ethanol g(-1) dry wt. cell h(-1) and 2.6 g sugar g(-1) dry wt. cell h(-1), respectively, at a dilution rate of 1.5 h(-1).
将酿酒酵母ATCC 24553的细胞固定在κ-卡拉胶中，并装填到锥形玻璃柱反应器中，用于在30℃和pH 4.5的条件下，从菠萝罐头厂废料生产乙醇。在稀释率为1.5 h⁻¹时，最大生产力为42.8 g乙醇·L⁻¹·h⁻¹。固定化细胞的体积乙醇生产力约为游离细胞的11.5倍。固定化细胞反应器在稀释率为1.0 h⁻¹的条件下运行了87天，固定化细胞活性没有任何损失。在稀释率为1.5 h⁻¹时，固定化细胞的最大比乙醇生产力和比糖摄取率分别为1.2 g乙醇·g⁻¹干重细胞·h⁻¹和2.6 g糖·g⁻¹干重细胞·h⁻¹。
