体外RNA合成的染色质模板
Chromatin as a template for RNA synthesis in vitro.
作者信息
Groner Y, Monroy G, Jacquet M, Hurwitz J
出版信息
Proc Natl Acad Sci U S A. 1975 Jan;72(1):194-9. doi: 10.1073/pnas.72.1.194.
Abstract
RNA transcribed in vitro from myeloblast chromatin by exogenously added RNA polymerase B predominantly consists of short chains that remain in hybrid structure with the template; the remainder of the product is free RNA of heterogeneous size. Addition of polyanions during synthesis caused an increase in the size and amount of free RNA with a concomitant decrease in the proportion of small RNA. The large molecular weight RNA is derived from the short RNA chains, which are synthesized de novo during the reaction in vitro. The effect of polyanions on the size and nature of the product may be related to structural changes induced in the template rather than to an inhibition of nuclease activity.
摘要
通过外源添加的RNA聚合酶B在体外从成髓细胞染色质转录而来的RNA主要由与模板保持杂交结构的短链组成；产物的其余部分是大小各异的游离RNA。合成过程中添加聚阴离子会导致游离RNA的大小和数量增加，同时小RNA的比例降低。大分子RNA源自短RNA链，这些短链是在体外反应过程中重新合成的。聚阴离子对产物大小和性质的影响可能与模板中诱导的结构变化有关，而不是与核酸酶活性的抑制有关。
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