猿猴病毒40序列在SV3T3染色质中的体外转录。
In vitro transcription of simian virus 40 sequences in SV3T3 chromatin.
作者信息
Astrin S M
出版信息
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2304-8. doi: 10.1073/pnas.70.8.2304.
Abstract
The template properties of chromatin were studied in a system in which transcription of unique genes could be examined. Chromation and DNA were isolated from SV3T3 cells, a line of simian virus 40 (SV40)-transformed mouse cells, and used as templates for Escherichia coli RNA polymerase. The SV40 sequences in the RNA transcribed in vitro and in RNA isolated from the transformed cells were analyzed by hybridization to radioactive RNA transcribed from SV40 DNA. The results indicate that the same sequences of the SV40 "-" strand (DNA strand which serves as a template for early RNA) are transcribed in vitro from chromatin as are found in transformed cells. In contrast, when the DNA isolated from SV3T3 chromatin by removal of proteins is used as a template, the entire SV40 "-" strand is transcribed. It is concluded that the specific template restriction observed with chromatin is due to the presence of proteins associated with the DNA.
摘要
在一个能够检测独特基因转录的系统中，对染色质的模板特性进行了研究。从猿猴病毒40（SV40）转化的小鼠细胞系SV3T3细胞中分离出染色质和DNA，并将其用作大肠杆菌RNA聚合酶的模板。通过与从SV40 DNA转录的放射性RNA杂交，分析体外转录的RNA以及从转化细胞中分离的RNA中的SV40序列。结果表明，在体外从染色质转录的SV40“-”链（作为早期RNA模板的DNA链）的序列与在转化细胞中发现的序列相同。相比之下，当通过去除蛋白质从SV3T3染色质中分离的DNA用作模板时，整个SV40“-”链都被转录。得出的结论是，观察到的染色质特异性模板限制是由于与DNA相关的蛋白质的存在。
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