质粒突变体的间接选择：在大肠杆菌中自我维持有缺陷的ColVBtrp突变体的分离
Indirect selection for plasmid mutants: isolation of ColVBtrp mutants defective in self-maintenance in Escherichia coli.
作者信息
Koyama A H, Wada C, Nagata T, Yura T
出版信息
J Bacteriol. 1975 Apr;122(1):73-9. doi: 10.1128/jb.122.1.73-79.1975.
Abstract
An efficient method for isolation of a large number of plasmid mutants is described. It is based on the fact that N-methyl-N'-nitro-N-nitrosoguanidine induces a number of closely linked mutations within a short segment of the bacterial chromosome. Thus, selection for reversions of an auxotrophic marker located on the ColVBtrp plasmid yielded a large fraction (more than 50 percent) of mutants defective in some plasmid functions, including its own maintenance in the host bacteria. The results of preliminary characterization of strains carrying these mutated plasmids are presented.
摘要
本文描述了一种分离大量质粒突变体的有效方法。该方法基于这样一个事实，即N-甲基-N'-硝基-N-亚硝基胍能在细菌染色体的短片段内诱导产生多个紧密连锁的突变。因此，通过选择位于ColVBtrp质粒上的营养缺陷型标记的回复突变体，获得了很大一部分（超过50%）在某些质粒功能上有缺陷的突变体，包括其在宿主细菌中的自身维持功能。文中还给出了携带这些突变质粒的菌株的初步鉴定结果。
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