Schukin N N
J Bacteriol. 1981 Sep;147(3):1015-20. doi: 10.1128/jb.147.3.1015-1020.1981.
Plasmid ColVBtrp maintenance in Erwinia carotovora cells was followed by measuring kinetics of elimination of plasmid genetic markers and loss of plasmid deoxyribonucleic acid. An E. carotovora mutant stably carrying plasmid ColVBtrp was isolated. Besides stable plasmid maintenance, the mutant showed altered sensitivity to male-specific phage MS2, sensitivity to drugs, and colony morphology.
通过测定质粒遗传标记消除动力学和质粒脱氧核糖核酸的丢失情况,追踪了质粒ColVBtrp在胡萝卜软腐欧文氏菌细胞中的维持情况。分离出了一个稳定携带质粒ColVBtrp的胡萝卜软腐欧文氏菌突变体。除了稳定的质粒维持外,该突变体对雄性特异性噬菌体MS2的敏感性、对药物的敏感性以及菌落形态都发生了改变。