Sampaio J L, Artiles N, Pereira R M, Souza J R, Leite J P
Lamina Laboratory, Rio de Janeiro, RJ, Brazil.
Braz J Infect Dis. 2001 Dec;5(6):352-5. doi: 10.1590/s1413-86702001000600010. Epub 2003 Feb 21.
Mycobacterium simiae is usually an environmental contaminant rarely associated with human disease. We report a fatal case of M.simiae infection in a 37 year old, HIV positive, male from whom the organism was isolated from blood culture. The identification of M.simiae was performed using DNA amplification followed by analysis on 3% agarose gel of the amplicon fragments after digestion by restriction endonucleases. The precise identification of mycobacterial isolates to the species level is important, with both epidemiological and therapeutic implications.
猿猴分枝杆菌通常是一种环境污染物，很少与人类疾病相关。我们报告了一例37岁HIV阳性男性的致命猿猴分枝杆菌感染病例，该菌从其血培养中分离得到。采用DNA扩增法鉴定猿猴分枝杆菌，扩增产物片段经限制性内切酶消化后，在3%琼脂糖凝胶上进行分析。将分枝杆菌分离株精确鉴定到种水平很重要，具有流行病学和治疗学意义。
